2型、5型腺病毒DNA与12型腺病毒DNA、大肠杆菌DNA序列的对比研究

目的通过对2型、5型人腺病毒DNA（Adv2、5 DNA）与Adv12 DNA及大肠杆菌DNA（EC DNA）序列进行对比研究，确定Adv2、5 DNA中能够拈抗细菌DNA的可能的活性片段——抑制性CpG寡核苷酸（CpG-N ODN）。方法从NCBI核酸数据库中获取Adv2、5、12 DNA和EC DNA序列，采用DNATools、BioEdit等软件对Adv2、5、12 DNA和EC DNA进行序列分析和比较，确定Adv2、5 DNA特征性的CpG基序，查找出Adv2、5 DNA中以上述CpG基序为核心的12碱基寡核苷酸（12-ODN）。结果确定了19种Adv2、5 DNA特征性的CpG基序，Adv2、5 DNA中以这19种CpG基序为核心的12-ODN共504条。结论504条12-ODN可能是Adv2、5 DNA的CpG-N ODN。

Comparison of sequence between adenoviral DNA and Escherichia coli DNA

Objective To identify the putative CpG-N ODNs in adenovisus 2 DNA (Adv2 DNA) and Adv5 DNA by comparing the sequence difference among Adv2, 5, 12 DNA and E.coli (EC) DNA. Methods Sequences of Adv2, 5, 12 DNA and EC DNA were obtained from the Entrez Nucleotides database at NCBI. The specific CpG motifs of Adv2 DNA and Adv5 DNA were identified after above sequences were analyzed and compared by softwares such as DNATools, BioEdit, and so on. All the 12-ODNs with specific CpG motif core were searched from Adv2 DNA and Adv5 DNA. Results 19 specific CpG motifs were ascertained and 504 12-ODNs were detected in Adv2 DNA and Adv5 DNA. Conclusion 504 12-ODNs were putative CpG-N ODNs in Adv2 DNA and Adv5 DNA.