| 熊果酸诱导人肝癌BEL-7404细胞凋亡的作用及其机制 |
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| 引用本文： | 王辉,方全华,李海军.熊果酸诱导人肝癌BEL-7404细胞凋亡的作用及其机制[J].肿瘤基础与临床,2010,23(2):93-95. |
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| 作者姓名： | 王辉 方全华 李海军 |
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| 作者单位： | 新疆医科大学附属肿瘤医院肝胆胰外科,新疆乌鲁木齐,830011 |
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| 基金项目： | 新疆维吾尔自治区卫生厅青年科技人才基金 |
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| 摘 要： | 目的 探讨熊果酸(UA)对人肝癌BEL-7404细胞凋亡的诱导作用及其机制.方法 采用MTT法检测UA对BEL-7404细胞增殖的抑制作用,流式细胞术检测BEL-7404细胞凋亡率,Western blot法检测BEL-7404细胞proeaspase-3、caspase-9、survivin蛋白的表达.并用顺铂(DDP)作为阳性对照.结果 UA和DDP对BEL-7404细胞增殖具有明显抑制作用,并呈浓度、时间依赖性;作用48 h后,阴性对照组、uA 50μmoL/L组、DDP 10 mg/L组的BEL-7404细胞凋亡率分别为(0.83±0.25)%、(16.10±0.30)%、(31.87±0.65)%,差异有统计学意义(P<0.01);作用48 h后,与阴性对照组相比,UA 50μmol/L组、DDP 10 mg/L组的procaspase-3、survivin蛋白表达降低,caspase-9蛋白表达升高,差异有统计学意义(P<0.01).结论 UA能够诱导BEL-7404细胞凋亡,其作用机制与procaspase-3、survivin蛋白表达降低及caspase-9蛋白表达升高有关.
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| 关 键 词： | 熊果酸 人肝癌细胞 凋亡 |
The Apoptosis-Inducing Effect of Ursolic Acid on Human Hepatocellular Carcinoma BEL-7404 Cells and Its Mechanism |
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| Wang Hui,Fang Quanhua,Li Haijun.The Apoptosis-Inducing Effect of Ursolic Acid on Human Hepatocellular Carcinoma BEL-7404 Cells and Its Mechanism[J].journal of basic and clinical oncology,2010,23(2):93-95. |
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| Authors: | Wang Hui Fang Quanhua Li Haijun |
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| Institution: | Wang Hui,Fang Quanhua,Li Haijun(Department of Hepatopancreatobiliary Surgery,the Affiliated Tumor Hospital,Xinjiang Medical University,Urumqi 830011,China) |
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| Abstract: | Objective To investigate the apoptosis-inducing effect of ursolic acid(UA) on human hepatocellular carcinoma BEL-7404 cells and its mechanism.Methods The inhibiting effect of UA on proliferation of BEL-7404 cells was evaluated by MTT assay.The apoptosis rate of BEL-7404 cells was determined by flow cytometry.The expression levels of procaspase-3,caspase-9 and survivin protein were detected by Western blot method.DDP was used as positive control.Results UA and DDP had the significant anti-proliferation effec... |
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| Keywords: | ursolic acid human hepatocellular carcinoma cells apoptosis |
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