| 一种适于ISSR分析的太子参DNA提取方法 |
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| 引用本文： | 王晓多,王晓理. 一种适于ISSR分析的太子参DNA提取方法[J]. 中国医药科学, 2012, 0(15): 56-57 |
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| 作者姓名： | 王晓多 王晓理 |
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| 作者单位： | 贵阳市花溪区农业局;贵阳市乌当农技服务中心 |
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| 摘 要： | 目的获得能用于中药太子参简单序列重复区间DNA标记技术等分析研究的高质量DNA。方法以太子参为材料,采用改良CTAB法提取太子参DNA,经紫外分光光度计和琼脂糖凝胶电泳检测。结果改良的CTAB法能获得高质量的太子参DNA,OD260/OD280>1.8,蛋白质、多糖、RNA等去除较彻底,适于简单序列重复区间分析。结论改良的CTAB法所提取太子参DNA适于简单序列重复区间分析。
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| 关 键 词： | 太子参 DNA OD值 |
An extraction method of genome DNA from Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.scape for ISSR analysis |
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| WANG Xiaoduo,WANG Xiaoli. An extraction method of genome DNA from Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.scape for ISSR analysis[J]. China Medicine and Pharmacy, 2012, 0(15): 56-57 |
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| Authors: | WANG Xiaoduo WANG Xiaoli |
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| Affiliation: | 1.Huaxi Agricultural Bureau of Guiyang City,Guiyang 550025,China;2.Wudang Agricultural Technique Service Center of Guiyang City,Guiyang 550018,China |
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| Abstract: | Objective To extract high quality DNA from Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.for ISSR and other molecular biological analysis.Methods It was examined with ultraviolet spectrophotometer and agarose electrophoresis that the DNA was extractied by improved CTAB.Results The improved CTAB method was used to extract DNA.The purity of the extracted DNA was very high with OD260/OD280 values > 1.8.The protein,polyhexose and RNAwere elminated completely.Conclusion The improved CTAB method was suitable for extracting DNA from Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm.for ISSR analysis. |
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| Keywords: | Pseudostellaria heterophylla(Miq.)Pax ex Pax et Hoffm. DNA OD value |
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