PTEN及PCNA在胃癌中的表达及其意义

目的探讨PTEN和PCNA在胃癌组织中的表达及其与临床病理特征的关系。方法采用免疫组化SP技术检测89例胃癌组织中PTEN和PCNA表达。结果胃癌组织中PTEN阳性表达率31.5%(28/89),与肿瘤直径、肿瘤的分化程度、浸润深度、淋巴结转移和肿瘤分期显著负相关。PCNA阳性表达率分别为69.7%(62/89),与肿瘤大小、肿瘤的分化程度、浸润深度、淋巴结转移和肿瘤分期显正相关。胃癌组织中PTEN与PCNA表达显著负相关,与病人预后相关,Kaplan-Meier生存曲线显示PTEN高表达者术后累计生存率显著高于低表达者;PCNA阳性表达者术后累计生存率显著低于阴性表达者。结论 PCNA,PTEN与胃癌侵袭转移能力和预后密切相关,两者是临床评价胃癌恶性程度的重要指标,联合检测PTEN和PCNA有助于判断胃癌预后。

Expression and significance of PCNA and PTEN in patients with gastric cancer

Objective To explore the PTEN,PCNA expression of gastric cancer and its relationship with clinical pathological characteristics.Methods By immunohistochemistry(SP)to detect PTEN,PCNA expression in 89 cases with gastric cancer and 12 cases of normal gastric tissues.Results The positive rate of PTEN in gastric cancer was 69.7%(62/89),significantly higher than normal gastric group(P<0.01);PCNA positive rate was 69.7%(62/89),significantly lower than the normal gastric group(P<0.01).Expressions of PTEN and PCNA closely related with diameter of tumor,differentiation of tumor,depth of tumor infiltration,lymph node metastasis and TNM stage was(P<0.05).PTEN expression was significantly negatively correlated(P<0.05),however PCNA expression was significantly positive correlated(P<0.05).Kaplan-Meier survival curves showed that cumulative survival rates of patients with positive expression of PTEN was significantly higher than those with negative expression.But cumulative survival rates of patients with positive expression of PCNA were significantly lower than those with negative expression.Conclusions The expressions of PTEN and PCNA have closely relationship with the invasion,metastasis and prognosis in patients with gastric tumor.Both were the important factors that determined the malignant degree of gastric cancer.Combined detection of PTEN and PCNA helps to determine the prognosis of gastric cancer.