Induction of cyclooxygenase protein and stimulation of prostaglandin E2 release by epidermal growth factor in cultured guinea pig gastric mucous cells |
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| Authors: | Osamu Nakano MD PhD Dr Choitsu Sakamoto MD PhD Kohei Matsuda MD PhD Yoshitaka Konda MD PhD Takashi Matozaki MD PhD Hogara Nishisaki MD PhD Ken Wada MD PhD Toshiya Suzuki MD Toru Uchida MD Munehiko Nagao MD PhD Masato Kasuga MD PhD |
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| Institution: | (1) Second Department of Internal Medicine, Kobe University School of Medicine, Kusunoki-cho, Chuo-ku, 650 Kobe, Japan |
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| Abstract: | The present study was undertaken to investigate whether epidermal growth factor (EGF) could stimulate prostaglandin E2 release, and if so, by what mechanism EGF would exert such an effect in gastric mucosal cells. In cultured guinea pig gastric mucous cells, EGF dosedependently stimulated prostaglandin E2 release, with maximal stimulation observed at 10 ng/ml. EGF stimulated an increase in cyclooxygenase activity, which was reduced by protein synthesis inhibitor, actinomycin D, and cycloheximide. EGF also stimulated the enzyme protein synthesis estimated by Western blot analysis, whereas EGF did not stimulate phospholipase A2 activity. These results suggest that such an effect of EGF onde novo synthesis of cyclooxygenase protein and prostaglandin E2 release may be involved at least in part in the mechanism of EGF-induced local regulation of gastric mucosal integrity. |
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| Keywords: | gastric mucosal cells epidermal growth factor prostaglandin E2 cyclooxygenase activity |
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