p53、c-erbB2基因、增殖细胞核抗原表达与卵巢上皮性癌预后的关系

背景与目的：p53、c-erbB2基因和增殖细胞核抗原（proliferating cell nuclear antigen,PCNA)的表达与卵巢癌预后关系问题已有报道,由于研究方法、样本大小等方面的不同,结果存在差异。为进一步探讨p53、c-erbB2基因和PCNA表达与卵巢上皮性癌预后的关系,我们进行了本研究。材料与方法：1990年3月1日至1994年3月31日间确诊的卵巢上皮性癌共84例,采用标记的链白素－生物素辣根过氧化酶复合物（LSAB）免疫组化方法检测癌组织中p53、c-erbB2基因和PCNA的表达,使用SPSS8．0版分析它们的表达与卵巢癌患者平均生存期、5年生存率之间的关系。结果：本组84例癌组织中,p53、c-erbB2和PCNA表达的阳性率分别为58．3％（49／84）、77．5％（65／84）和100％（84／84）。单因素分析显示,c-erbB2过度表达和PCNA表达强度与患者的平均生存期、5年生存率均呈负相关（P值分别为0．05和小于0．01）,未发现p53蛋白表达与患者的平均生存期、5年生存率之间存在相关性（P＞0．05）。多因素分析发现卵巢上皮性癌的预后与临床分期、组织分化程度有关,而与p53、c-erbB2和PCNA的表达均无相关性。结论：p53的表达与卵巢上皮性癌的预后无关,而c-erbB2、PCNA表达对卵巢上皮性癌预后的影响是间接的。临床分期、组织分化程度仍是卵巢上皮性癌独立的预后因素。

Relationship between expressions of p53, c-erbB2 genes,proliferating cell nuclear antigen and prognosis of patients with ovarian epithelial carcinoma

Background &Objective:There were a few reports about the relationship between the expressions o f p53,c-erbB2Genes,and proliferating cell nuclear antigen(PCNA)with the prognosis from the patients with ovarian epithelial carcinoma (EOC).But conclusions were difference because of various methods and samples.This study was designed to investigate the relatio nship between the expressions of p53,c-erbB2Genes,and PCNA with the pro gnosis from the patients with EOC.Materials &Methods:A total of 84female patients with EOC were enrolled in this study,who were hospitalized in Department of Gynecology,Cancer Center,Sun yat-s en University from March 11990to March 311994.The expressions of p53,c-erbB-2,and PCNA from the tumor samples we re detected by Labelling-streptavidin-biotin(LSAB)immunohistochemistry assay.The re lationship between the expression o f the above marks and the prognosis of the patients were then analyzed.Statis tical analyses were performed using SPSS 8.0softwave.Results:Forty-nine tumor samples(58.3%)exhibited p53overexpression,65(77.4%)exhibited c-erbB2overexpression,and all(100%)exhibited PCNA overexpression.In univariate analysis,there was marginally significant difference in the mean survival,the 5years surv ival between the patients exhibited no c-erbB2overexpression and the patients exh ibited any c-erbB2overexpression(P=0.05).There was significant difference i n the mean survival,the 5years survival b etween the patients showed lower exp ression of PCNA and those showed high er expression of PCNA(P<0.01),and there was no significant difference in the mean survival,the 5years survival between the patients with overexpression of p53and without overexpression of p53(P>0.05).In multivariate analysis,only sta ge and histological grade were the important significant prognostic facto rs of the patients with EOC,and there was no statistical significance between the overexpre ssion of c-erbB-2and PCNA with the pr ognosis.Conclusions:There was no significant correlation between the overexpression of p53gene and the prognosis of t he patients with EOC.The expression of c-erbB2or PCNA was not an independent prognostic factor for prognosis of the patien ts with EOC.Stage and histological g rade were still the independent significant prognostic factors of EOC.