生命早期应激小鼠成年后免疫功能的改变

目的 观察生命早期应激对小鼠成年后免疫功能的影响.方法 生命早期应激采用新生鼠母爱剥夺和孤养两种方式,分别在小鼠1,2和3月龄检测外周血白细胞比例、刀豆蛋白A(Con A)和脂多糖(LPS)诱导的脾淋巴细胞转化以及外周血清对正常小鼠类别细胞增殖的影响.结果 生命早期母爱剥夺小鼠成年后外周血中性粒细胞比例由(17.94±1.24)%,(17.33±1.80)%和(21.25±2.82)%分别升高至1,2,3月末的(27.17±3.19)%,(29.75±2.76)%和(28.33±3.12)%;淋巴细胞比例降低.由(79.06±1.18)%,(77.83±3.05)%和(72.46±4.45)%分别降低至1,2,3月末的(69.72±4.97)%,(64.00±3.9)%,(66.74±2.67)%.Con A和LPS诱导的脾淋T和B巴细胞转化增强,外周血清对正常淋巴细胞增殖具有显著的抑制作用.结论 生命早期应激可导致小鼠成年后细胞性免疫功能亢进.

Changes of peripheral immunity in adult mice with early life stress

Objective To investigate the effect of early life stress on peripheral immunity in adult in mice. Methods The neonatal mice were maternal seperation 2 hours everyday from day 2~(nd) to 21~(st), and breeding alone after day 22~(nd). The differential white blood cell count were conducted with blood smear. Concanavalin (Con A) and lipopolysaccharide( LPS) induced splentic proliferation were measured with MTT method, and the effect of serum from stressed mouse on normal lymphocte proliferation were also tested with MTT. Results Adult mice with early life stress showed that neutrophile granulocytes increased from (17. 94 ±1.24)%,( 17. 33 ± 1.80)% and (21.25 ±2.82)% to (27.17 ±3.19)% ,(29.75 ±2.76)% and (28.33 ±3.12)% at 1,2 and 3 months old. Lymphocytes decreased from (79. 06 ± 1. 18 )%, (77. 83 ± 3. 05 )% and (72. 46 ± 4. 45 )% to (69.72 ±4.97)% ,(64.00 ±3.9)% and (66.74 ±2.67)% at 1,2 and 3 months old,and monocyte increased as well. Both Con A and LPS induced splentic proliferation increased in mice with early life stress at 1,2 and 3 months old. Sera from mice with early life stress decreased the normal lymphocyte proliferation compared with sera from control mice. Conclusion Early life maternal deprivation increase cellular immunity in adulthood in mice.