鲜壁虎醇提物与水提物体内、外对肿瘤活性的影响

目的:研究鲜壁虎水提物与醇提物体内、外的抗肿瘤活性。方法:体外采取MTT法测定鲜壁虎水提物、醇提物对BEL-7402细胞和HepG2细胞生长的抑制作用。体内复制移植瘤小鼠H22模型,给药后计算抑瘤率、胸腺指数和脾脏指数。结果:0.0625mg·mL-1壁虎水提物对HepG2细胞生长有显著抑制作用(P<0.05),1.0000、0.2500、0.0625mg·mL-1壁虎水提物对BEL-7402细胞的生长均有显著抑制作用(P<0.05)。1.0000、0.2500、0.0625mg·mL-1壁虎醇提物对2种细胞有促进增殖的趋势。1.0000、0.2500、0.0625mg·mL-1壁虎水提物与1.0000、0.0625mg·mL-1壁虎醇提物均可显著抑制H22荷瘤小鼠肿瘤生长活性(P<0.05)。结论:鲜壁虎水提物体外有抑制肿瘤细胞生长的作用,醇提物有促进细胞增殖的趋势。鲜壁虎水提物、醇提物体内均有抗肿瘤活性,水提物抑瘤率高于醇提物。

Effects on Tumor Activity of Aqueous and Ethanol Extracts from Gekko swinhonis Gunther

OBJECTIVE:To study the in vitro and in vivo antitumor activity of the aqueous and ethanol extracts from Gekko swinhonis Gunther.METHODS:The inhibitory effects of aqueous and ethanol extracts from G.swinhonis on proliferation of BEL-7402 cell and Hep G2 cell in vitro were measured by MTT assay.The in vivo H22 tumor mice model was induced to calculate anti-tumor rate,thymus gland index and spleen index.RESULTS:The aqueous extracts of G.swinhonis Guther 0.062 5 mg·mL-1 inhibited the proliferation of Hep G2 cells.The aqueous extracts of G.swinhonis Guther 1.000 0,0.250 0,0.062 5 mg·mL-1 inhibited the proliferation of BEL-7402 cells.The water extracts from G.swinhonis Guther 1.000 0,0.250 0,0.062 5 mg·mL-1 and the ethanol extracts from G.swinhonis Guther 1.000 0,0.062 5 mg·mL-1 promoted the proliferation of 2 kinds of cells.The ethanol extracts from G.swinhonis Guther 1.000 0,0.250 0,0.062 5 mg·mL-1 inhibited the activity of tumor in H22 hepatocarcinoma-bearing mice.CONCLUSION:The agueeus extracts of G.swinhonis Guther can inhibit the growth of tumor cell in vitro,while ethanol extract of it promote the proliferation of tumor cell.They all have antitumor effect,but the in vivo antitumor effects of aqueous extracts of G.swinhonis Guther is more effective than ethanol extracts.