| 深圳地区麻疹病毒流行株血凝素蛋白H基因及核蛋白N基因序列分析 |
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| 引用本文： | 陈传德,陈伟红,卓菲,刘卫民. 深圳地区麻疹病毒流行株血凝素蛋白H基因及核蛋白N基因序列分析[J]. 实用预防医学, 2007, 14(4): 996-997 |
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| 作者姓名： | 陈传德 陈伟红 卓菲 刘卫民 |
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| 作者单位： | 深圳市罗湖区疾病预防控制中心,广东,深圳,518020 |
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| 基金项目： | 深圳市卫生科技立项(200204166) |
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| 摘 要： | 目的探讨深圳地区目前流行的麻疹病毒的基因型别和特征。方法采用B95a细胞分离培养麻疹病毒,通过RT-PCR方法扩增麻疹病毒核蛋白基因C末端450bp片段和血凝素H基因全序列并测序,其序列与Genebank中国内外流行的麻疹病毒8个基因组的代表毒株的序列进行同源性分析。结果分离到1株麻疹病毒,N基因与H1基因型代表毒株相比同源性为98.4%,H基因与H1基因型代表毒株相比同源性为99.5%。结论深圳市麻疹流行毒株属于H1基因型。
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| 关 键 词： | 麻疹病毒 核蛋白N基因 血凝素H基因 基因型 序列分析 |
| 文章编号： | 1006-3110（2007）04-0996-02 |
| 收稿时间： | 2007-03-27 |
| 修稿时间： | 2007-03-27 |
Sequence Analysis of Nucleoprotein(N) Gene and Hemagglutinin(H) Gene of Mealses Virus Isolated in Shenzhen City |
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| CHEN Chuan - de, CHEN Wei - hong , ZHUO Fei ,et al.. Sequence Analysis of Nucleoprotein(N) Gene and Hemagglutinin(H) Gene of Mealses Virus Isolated in Shenzhen City[J]. Practical Preventive Medicine, 2007, 14(4): 996-997 |
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| Authors: | CHEN Chuan - de CHEN Wei - hong ZHUO Fei et al. |
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| Affiliation: | Center for Disease Control and Prevention of Luohu District, Shenzhen 518020, Guangdong , China |
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| Abstract: | Objective To explore the genetic characteristic and genotype of measles viruses circulating in Shenzhen City.Methods Throat swabs were collected for virus isolation.A 450bp fragment of the C-terminal of the nucleoprotein(N) gene and the full sequence of hemagglutinin(H) gene were amplified by RT-PCR and subjected to sequence analysis by using DNAstar software.Results Only 1 strain of measles virus was isolated from the 15 throat-swabs.N gene homogeneity was 98.4% and H gene homogeneity was 99.5% when compared with other wild-type viruses of H1 genotype.Conclusion Measles viruses circulating in Shenzhen belong to the H1 genotype. |
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| Keywords: | Measles virus Nucleoprotein gene Hemagglutinin gene Genotype Sequence analysis |
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