Cyclin D1在胶质瘤细胞系中表达分布模式的初步研究 |
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引用本文: | 黄安炀,章翔,曹云新,李霞,刘新平.Cyclin D1在胶质瘤细胞系中表达分布模式的初步研究[J].第四军医大学学报,2003,24(22):2024-2026. |
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作者姓名: | 黄安炀 章翔 曹云新 李霞 刘新平 |
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作者单位: | 1. 第四军医大学,西京医院全军神经外科研究所,陕西,西安,710033 2. 第四军医大学,基础部免疫学教研室,陕西,西安,710033 3. 第四军医大学,基础部生物化学教研室,陕西,西安,710033 |
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摘 要: | 目的:探讨cyclin D1在胶质瘤细胞系中的细胞周期表达模式,将为从生物学功能上阐明cyclin D1在胶质瘤细胞周期演进中的作用.方法:利用流式细胞仪双参数法和细胞同步化,确定cyclin D1在胶质瘤细胞系SHG-44和BT-325中的细胞周期表达分布模式.结果:在胶质瘤细胞系SHG-44和BT-325中,Cyclin Dl的表达呈细胞周期依赖性:在SHG-44中,cyclin D1在G1期表达最高,4h左右达到峰值,S期及G2/M期下降,但仍可检测到;在BT-325中,cyclin D1在G1期表达最高,6h左右达到峰值,S期及G2/M期下降,但仍可检测到.结论:cyclin D1蛋白质的表达呈细胞周期依赖性,且与细胞周期行进有关;cyclin D1在SHG-44和BT-325胶质瘤细胞进入G1期的早期发挥着重要的生物学作用。
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关 键 词: | 神经胶质瘤 细胞周期蛋白D1 cyclinD1 细胞周期表达模式 细胞周期依赖性 |
文章编号: | 1000-2790(2003)22-2024-03 |
修稿时间: | 2003年9月8日 |
Primary study on cyclin D1 expression pattern in human glioma cell line by double parameter flow cytometry |
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Abstract: | AIM: To elucidate cyclin D1 expression model in human glioma cell lines SHG 44 and BT 325. METHODS: Cyclin D1 expression model of glioma cell lines SHG 44 and BT 325 was analyzed by using a double parameter flow cytometry after cell synchronization. RESULTS: Double parameter flow cytometry showed that cyclin D1 expression in human glioma cell lines SHG 44 and BT 325 was in a cell cycle dependent manner and was oscillated with cell cycle, reaching the peak in the G1 phase, decreasing in the S phase and being detectable in the G2/M phase. CONCLUSION: Cyclin D1 expression in glioma cell lines SHG 44 and BT 325 is in a cell cycle dependent manner and has a periodical oscillation with cell cycle, indicating that cyclin D1 plays an important role in the progression of G1/S phase in SHG 44 and BT 325. |
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Keywords: | glioma cyclin D1 cell cycle |
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