| 抗癌胚抗原单克隆抗体可变区基因的克隆及鼠-人嵌合抗体基因在E.coli中的高效表达 |
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| 引用本文： | 李彪,朱承谟,余拥军,姚潇怡,吴祥甫. 抗癌胚抗原单克隆抗体可变区基因的克隆及鼠-人嵌合抗体基因在E.coli中的高效表达[J]. 现代免疫学, 1998, 0(3) |
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| 作者姓名： | 李彪 朱承谟 余拥军 姚潇怡 吴祥甫 |
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| 作者单位： | 上海第二医科大学附属瑞金医院!上海200025(李彪，朱承谟)，中国科学院上海生物化学研究所!上海200031(余拥军，姚潇怡，吴祥甫) |
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| 基金项目： | 1995年上海市教委博士点基金（NO.39770231），国家自然科学基金资助 |
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| 摘 要： | 本文采用反转录-聚合酶链反应（RT-PCR）技术,从抗癌胚抗原（CEA）单克隆抗体杂交瘤细胞中克隆到了该抗体的重、轻链可变区（Ⅴ区）基因,并分别将其与人的恒定区基因 C_(3γ)、C_κ相拼接,构建鼠-人嵌合抗体基因。SDS-PAGE和Western-Blot分析结果证实嵌合重、轻链抗体基因在E.coli中得到高效表达,表达量约为菌体可溶蛋白的30％和15％。放射免疫分析法（RIA）和间接ELISA测定的结果表明嵌合重链基因表达产物具有与CEA结合的能力。
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| 关 键 词： | 癌胚抗原抗体基因 嵌合抗体基因 基因表达 |
The Cloning and Construction of a Murine-Human Chimeric Antibody with Specificity for Carcinoembryonic Antigen and the High Expression of the Chimeric Genes in E. coli |
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| Li Biao,Zhu Chengmo,Yu Yongjun,et al.. The Cloning and Construction of a Murine-Human Chimeric Antibody with Specificity for Carcinoembryonic Antigen and the High Expression of the Chimeric Genes in E. coli[J]. Current Immunology, 1998, 0(3) |
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| Authors: | Li Biao Zhu Chengmo Yu Yongjun et al. |
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| Abstract: | The genes encoding the heavy and light chain variable region (VH and VK) of the murine monoclonal antibody with high specificity and affinity for carcinoembryonic antigen have been cloned by using RT-PCR. These genes were combined to the human Cr3 and Ck genes respectively to construct the murine-human chimeric antibody genes. The chimeric heavy and light chains were expressed in E.coli with high levels, the former reaching 30% of the total bacterial protein, while the latter 15%, and characterized by SDS-PAGE, Western blot, RIA and ELISA to demonstrate that the chimeric heavy chain has the CEA-binding ability. |
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| Keywords: | anti-CEA antibody gene chimeric antibody gent gene expression |
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