Derivation of hybrids between a thymoma line and spleen cells activated in a mixed leukocyte reaction |
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Authors: | G. K hler,I. Lefkovits,B. Elliott,A. Coutinho |
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Affiliation: | G. Köhler,I. Lefkovits,B. Elliott,A. Coutinho |
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Abstract: | We have established 16 independent hybrid lines derived from a fusion between an AKR thymoma and mixed leukocyte reaction-stimulated C57BL/6 spleen cells. The hybrids express both parental H-2 alleles and show hybrid glucose-6-phosphate isomerase activity. Two of the lines showed Fc receptor activity not expressed in the parental thymoma. The AKR Thy-1.1 allele was expressed in all hybrid lines, but no Thy-1.2 could be detected. No killing activity was retained in the hybrid cells, probably because Sendai virus-mediated fusion of killer cells resulted in the lysis of the thymoma fusion partner. |
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