实验性水上漂浮和高强度运动对大鼠下丘脑-垂体-睾丸轴功能的影响

目的:探讨大鼠在经历水上漂浮和高强度运动后血清T、皮质酮(CORT)、LH、FSH的变化规律。方法:将大鼠随机分为对照组(C组)、水上漂浮组(A组)、水上漂浮并高强度运动组(B组)3组,每组12只,实验分为3个阶段:第一阶段为适应性训练阶段,3d,每天10min,对模拟水上漂浮装置和大鼠跑台进行适应性训练;第二阶段为水上漂浮阶段,共3h;第三阶段为水上漂浮并高强度运动阶段,共2h。A组大鼠和B组大鼠分别经历第一、二阶段和第一、二、三阶段。实验结束后测定血清T、CORT、LH、FSH水平。结果:血清T(呈非正态分布,经对数转换后分析)A组为0.17±0.07,B组-1.11±0.35,与C组0.67±0.26相比均显著下降(P<0.05);LHB组为(3.97±1.57)mIU/ml,与C组(6.49±1.56)mIU/ml相比显著下降(P<0.05);FSH无显著变化;CORTB组为(977.22±207.36)ng/ml,与C组(434.58±110.45)ng/ml相比明显上升(P<0.05)。结论:在水上漂浮阶段,大鼠血清T出现明显下降,说明严重的心理应激会抑制下丘脑-垂体-睾丸轴的功能。随着应激原的增多和应激强度的增加,LH明显下降,T进行性下降,说明下丘脑-垂体-睾丸轴功能受到进一步抑制。

Experimental Navigation and Deuteroexhaustive Exercise Suppress the Function of the Hypothalamic-pituitary-testicle Axis in Rats

OBJECTIVE: To study the effects of experimental navigation and deuteroexhaustive exercise on the serum levels of testosterone (T), corticosterone (CORT), luteinizing hormone (LH) and follicle stimulating hormone (FSH). METHODS: Thirty-six male SD rats were randomly divided into an experimental navigation group (Group A), which underwent 180 min wearing floating with psychological stress, a deuteroexhaustive exercise group (Group B), which were subjected to 120 min intensive running on the treadmill after the accomplishment of the same procedure as Group A, and a control group (Group C). Blood samples were obtained at the end of the experiment to determine the T, CORT, LH and FSH of the rats. RESULTS: Compared with Group C, serum T was statistically decreased in Group A and B (P < 0.05), while CORT was increased slightly in Group A and significantly in Group B (P < 0.05). A statistically lower level of serum LH was observed in Group B (P < 0.05), but no significant differences were found in serum FSH among the three groups. CONCLUSION: Stresses of experimental navigation and intensive exercise suppress the function of the hypothalamic-pituitary-testicle axis in rats.