β-桧木醇/纳米氧化锌/聚己内酯纳米纤维的体外抑菌效果及生物相容性评价

目的 制备β-桧木醇/纳米氧化锌/聚己内酯(beta-hinokitiol/zinc oxide nanoparticles/polycaprolactone,β-H/ZnONPs/PCL)纳米纤维,评价其对耐药金黄色葡萄球菌(methicillin-resistant staphylococcus aureus,MRSA)的体外抑菌效果及生物相容性.方法 利用静电纺丝技术制备β-H/ZnONPs/PCL纳米纤维并通过扫描电镜观察,傅里叶红外光谱仪检测其光谱特征,同时检测其透气性、降解性能等指标,考察不同载药比例的纳米纤维对MRSA的体外抑菌效果.参照医疗器械生物学评价标准实施指南,初步研究其体外生物相容性.结果 在PCL纺丝液浓度(质量浓度)为16％、电压为14 kV、喷射速度为0.3 mL/h、接收距离为9 cm时制备的纳米纤维直径分布较为均匀,直径大多分布在150 ～ 350 nm,红外光谱显示各组纳米纤维在1 732 cm-1(C=0)、2 950 cm-1(C-H)附近有吸收峰.纳米纤维的透气性明显优于普通无纺布组,差异有统计学意义(P＜0.05).β-H/ZnONPs/PCL纳米纤维在PBS缓冲液(pH=7.5)中缓慢降解.ZnONPs制成纳米纤维后仍能增加β-H对MRSA的抑菌作用,且纳米纤维中ZnONPs(质量浓度)的含量为1％时,纳米纤维对MRSA的抑菌效果随β-n含量的增大而增强(P＜0.05),当β-H(质量浓度)含量为0.05％时,纳米纤维的抑菌效果并未随ZnONPs含量的变化而明显改变(P＞0.05).通过MTT法细胞毒性实验、细胞粘附性实验和溶血性实验表明0.05％ β-H/0.1％ ZnONPs/16％ PCL组成的纳米纤维具有较好的体外生物安全性.结论 0.05％ β-H/0.1％ZnONPs/16％ PCL组成的纳米纤维对MRSA有较好的体外抑菌效果,其体外生物相容性评价结果符合医疗器械生物学标准.

In vitro antibacterial effect and biocompatibility of nanofibers composed of beta-hinokitiol,zinc oxide nanoparticles and polycaprolactone

Objective To prepare the beta-hinokitiol/zinc oxide nanoparticles/polycaprolactone (β-H/ZnONPs/PCL) nanofibers,and evaluate its antibacterial effect on methicillin-resistant Staphylococcus aureus (MRSA) and biocompatibility.Methods β-H/ZnONPs/PCL) nanofibers were prepared with electrostatic spinning technology.Then the obtained nanofibers were observed with scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FTIR).Water vapor permeability and degradation were also detected for the fibers.The antibacterial effect of the nanofibers loaded with different doses of ZnONPs on MRSA was determined in vitro.The biological compatibility of the obtained nanofibers was evaluated with the reference of the guidelines.Results The prepared nanofibers had an uniform diameter (mainly 150 to 350 nm) under the condition of 16％ PCL (w/v),voltage of 14 kV,ejection speed of 0.3 mL/h,and receiving distance of 9 cm.FTIR showed that absorption peaks were observed at the 1 732 cm-1 (C =O) and 2 950 cm-1 (C-H).The water vapor permeability of the nanofibers was superior to the non-woven fabrics (P ＜ 0.05),and the nanofibers could be slowly degraded in the PBS (pH =7.5).The loaded ZnONPs in the nanofibers still increased the inhibitory effect of β-H on MRSA.When the ratio of ZnONPs was 1％ (w/v),β-H exerted antibacterial effect on MRSA in a dose-dependent manner (P ＜ 0.05).However,the antibacterial effect was not promoted with the increase of ZnONPs concentration under the ratio of 0.05％ β-H (w/v) (P ＞ 0.05).Cytotoxicity test (MTT assay),cell adhesion test and hemolytic test indicated that 0.05％ β-H/0.1％ ZnONPs/16％ PCL had proved biocompatibility.Conclusion Nanofibers of 0.05％ β-H/0.1％ ZnONPs/16％ PCL exert sound antibacterial effect on MRSA,and meet the biocompatible standards for medical devices and equipments.