淫羊藿苷对哮喘大鼠醌氧化还原酶1及谷胱甘肽还原酶表达及氧化应激的影响

目的探讨淫羊藿苷对哮喘大鼠醌氧化还原酶1(NQO1)及谷胱甘肽还原酶(GR)表达及氧化应激的影响。方法选取40只健康雌性Wistar大鼠,随机将其分为正常组、模型组、布地奈德组及淫羊藿苷组,每组10只。除正常组外,其余组大鼠采用卵蛋白溶液造成慢性大鼠哮喘模型。实验期间正常组及模型组每天灌胃生理盐水,淫羊藿苷组每天灌胃淫羊藿苷120 mL/kg,布地奈德组致敏2周后在卵蛋白溶液雾化吸入后给予布地奈德吸入。于末次激发后24 h内处死各组(正常组同期处死)大鼠并取出右肺组织,以ELISA方法检测各组氧化因子活性氧(ROS)表达水平,采用RT-PCR法检测各组GR mRNA表达水平,采用Westernblot方法检测各组NQO1蛋白表达水平。结果模型组大鼠肺组织中ROS表达水平明显高于正常组(P<0.05),GR mRNA及NQO1蛋白表达水平均明显低于正常组(P均<0.05)。淫羊藿苷组及布地奈德组大鼠肺组织中ROS表达水平均明显低于模型组(P均<0.05),GR mRNA及NQO1蛋白表达水平均明显高于模型组(P均<0.05),淫羊藿苷组与布地奈德组各指标比较差异均无统计学意义(P均>0.05)。结论淫羊藿苷可上调哮喘大鼠GR mRNA及NQO1蛋白的表达,减轻哮喘大鼠氧化应激反应。

Effect of icariin on the expression of NAD(P)H:quinine oxidoreductase 1, glutathione reductase and oxidative stress in asthmatic rats

objective It is to investigate the effect of icariin on the expression of [NAD(P)H:quinine oxidoreductase 1(NQO1] and(glutathione reductase(GR) and oxidative stress in asthmatic rats. Methods Forty healthy female Wistar rats were selected and randomly divided into normal group, model group, budesonide group and icariin group, each group had 10 rats.The rats in every group except for the normal group were treated with ovalbumin solution to establish chronic asthma models. During the experiment, the normal group and the model group were administrated with normal saline by gavage every day, the icariin group was administrated with icariin 120 mL/kg by gavage every day, and the budesonide group was given budesonide by aerosol inhalation after administration of ovalbumin solution solution after sensitization for 2 weeks. The rats in each group were sacrificed within 24 hours after the last simulation(normal group was sacrificed at the same time) and their right lung tissue was taken to detect the expression of oxidative factor reactive oxygen species(ROS)by ELISA method, and the expression of GR mRNA in each group was detected by RT-PCR method, the levels of NQO1 protein were detected by Westernblot method. Results The expression level of ROS in the lung tissue of the model group was significantly higher and the levels of GR mRNA and NQO1 protein were significantly lower than those of the normal group(all P<0.05). The expression levels of ROS in the lung tissue of the icariin group and budesonide group were significantly lower and the levels of GR mRNA and NQO1 protein were significantly higher than those of the model group(all P<0.05). There was no statistically significant difference in each index between the icariin group and budesonide group(all P>0.05). Conclusion Icariin can up-regulate the expression of GR mRNA and NQO-1 protein and reduce the level of oxidative stress in asthmatic rats.