PTEN、PI3K和miR-21在胃癌中的表达及相关性研究

目的 探讨第10号染色体同源丢失性磷酸酶-张力蛋白基因(PTEN)、磷脂酰肌醇3-激酶(PI3K)和miR-21在胃癌中的表达及相互关系.方法 收集77例胃癌患者手术切除的胃癌组织及胃黏膜组织标本,应用荧光定量RT-PCR及Western blot-ting技术检测miR-21、PTEN和PI3K蛋白的表达水平,并分析miR-21与PTEN/PI3K信号通路之间的关系,以及与临床病理参数之间的相关性.结果 胃癌组织中miR-21及PI3K蛋白的表达水平均明显高于胃黏膜组织(P<0.05),PTEN蛋白的表达水平则明显降低(P<0.05);胃癌组织中PTEN蛋白的表达与肿瘤浸润深度、分化、TNM分期、淋巴转移有关(P<0.05),PI3K蛋白的表达与肿瘤淋巴转移和TNM分期有关(P<0.05);胃癌组织中PTEN蛋白的相对表达水平与miR-21和PI3K蛋白表达呈负相关(r=-0.428,P<0.05;r=-0.517,P<0.05).结论 miR-21可能通过抑制PTEN,激活P13K信号通路参与胃癌的发生发展.

Expressions of PTEN,PI3K and miR-21 in gastric cancer and their correlations

Objective To investigate the expressions of phosphatase and tensin homologue deleted on chromosome 10(PTEN),phos-phatidylinositol 3-kinase(PI3K) and miR-21 in gastric cancer(GC) and normal gastric tissues and their correlation. Methods Using Western blotting and real-time quantitative polymerase chain reaction( RT-qPCR),we examined the expression of PTEN,PI3K and miR-21 in 77 cases of gastric cancer tissues and paired normal gastric tissues. Results Compared withnormal gastric tissues,the gastric cancer tissues showed a significantly higher expression of miR-21(t = 16. 7685,P < 0. 05) and PI3K(t = 11. 3991,P < 0. 05),but significantly lower PTEN expression(t = 18. 3813,P < 0. 05). The expression of PTEN protein in gastric cancer was highly correlated with the degree of differentiation,invasion depth,lymph node metastasis and TNM stage(P < 0. 05). PI3K protein expression was asso-ciated with TNM stage and lymph node metastasis(P < 0. 05). PTEN protein expression was negatively correlated with the expression of miR-21(r = - 0. 428,P < 0. 05) and PI3K protein(r = - 0. 517,P < 0. 05) in gastric cancer tissues. Conclusions miR-21 promotes gastric carcinogenesis via inhibiting PTEN expression which in turn upregulating the expression of P13K in gastric cancer cells.