Toll样受体2在子宫内膜异位症病人异位和正常内膜组织中的表达及意义

目的 探讨 Toll样受体2(TLR2)在子宫内膜异位症(EMs)病人异位和正常内膜组织中的表达和意义.方法 选取2014年2月-2016年2月在昆山市中医医院妇科经病理证实为子宫内膜异位症病人的异位内膜39例(EMs异位内膜组)以及在位内膜32例(EMs在位内膜组),再选取30例未患有子宫内膜异位的女性在位内膜组织30例(正常对照组).采用RT-PCR法检测TLR2在EMs异位内膜、EMs在位内膜以及正常内膜组织中mRNA的表达情况.采用 Western blot法检测TLR2在各组中蛋白的表达情况.结果 EMs异位内膜组、EMs在位内膜组、正常对照组中TLR2 mRNA和蛋白表达有显著性差异(P﹤0.05),EMs异位内膜组和EMs在位内膜组TLR2 mRNA和蛋白表达显著高于正常对照组,EMs异位内膜组中的TLR2 mRNA和蛋白表达显著高于EMs在位内膜组.结论 TLR2 mRNA和蛋白的高表达,说明TLR2在EMs的发病和发展过程中可能起着重要作用.

Expression and significance of TLR2 in ectopic and normal endometrium from patients with endometriosis

Objective To investigate the expression and significance of TLR2 in ectopic and normal endometrium from patients with endometriosis.Methods A total of 39 cases of ectopic endometrium (EMs ectopic endometrium group) and 32 cases of endometrium (EMs eutopic endometrium group) from endometriosis patients confirmed by pathology in traditional chinese medicine hospital of kunshan between February 2014 and February 2016,and 30 cases of endometrial tissue without endometriosis (normal control group) were selected.The mRNA expression of TLR2 in ectopic endometrium,eutopic endometrium and normal endometrium was detected by RT-PCR.Western blot was used to detect the expression of TLR2 protein in each group.Results There was significant difference in the mRNA and protein expression of TLR2 between EMs ectopic endometrium group,EMs eutopic endometrium group and normal control group (P<0.05).The expression of mRNA and protein in EMs ectopic and eutopic endometrium group was significantly higher than that in normal control group,and the expression in EMs ectopic endometrium group was significantly higher than the eutopic endometrium group.Conclusion The high expression of TLR2 indicates that TLR2 may play an important role in the pathogenesis and development of endometriosis.