Inhibition of mitochondrial function reduces DNA repair in human mononuclear cells期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Inhibition of mitochondrial function reduces DNA repair in human mononuclear cells

Authors:	Anat Gafter-Gvili Michal Herman Yaacov Ori Asher Korzets Avry Chagnac Boris Zingerman Benaya Rozen-Zvi Uzi Gafter Tsipora Malachi

Affiliation:	^a Department of Nephrology and Hypertension, Rabin Medical Center, Petah Tikva, Israel ^b Department of Hematology, Rabin Medical Center, Petah Tikva, Israel ^c Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel

Abstract:	Background Mitochondria provide ATP and Ca²⁺ needed for DNA repair, but also produce reactive oxygen species (ROS), which may damage DNA. Aim To investigate the effect of mitochondrial function inhibition on DNA repair. Method Five mitochondrial inhibitors acting at various sites of electron transport were studied. Human peripheral blood mononuclear cells, spontaneous and H₂O₂-indcued DNA repair, as well as %-double-stranded-DNA, were measured. Results All mitochondrial inhibitors suppressed spontaneous and H₂O₂-induced DNA repair. However, their effect on %-double-stranded-DNA differed, which is partly related to ROS suppression. Conclusion Mitochondrial inhibition may enhance efficacy and reduce toxicity of radiation and cytotoxic drugs therapy.

Keywords:	DNA breaks Spontaneous DNA repair H₂O₂-indcued DNA repair %-Double-stranded-DNA Mitochondrial inhibitors Human peripheral blood mononuclear cells 3H-thymidine incorporation
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