Chronic myelogenous leukemia cells convert to myofibroblasts in vitro: effect of vascular endothelial growth factor on development of the microenvironment |
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Authors: | Shirasaki Ryosuke Tashiro Haruko Mizutani-Noguchi Mitsuho Kawasugi Kazuo Shirafuji Naoki |
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Institution: | Department of Hematology and Oncology, Teikyo University School of Medicine, Tokyo, Japan |
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Abstract: | To elucidate the biological characteristics of chronic myelogenous leukemia (CML) cells, we observed morphological and functional changes of CML cells during primary long-term culture, in which their morphology changed to that of myofibroblasts with similar molecular characteristics to the parental CML cells including BCR-ABL fusion gene, and produced cytokines such as granulocyte colony-stimulating factor, interleukin-6, and vascular endothelial growth factor (VEGF)-A. When cultured on the CML-derived myofibroblasts, parental non-adherent CML cells significantly proliferated. When anti-VEGF-A-neutralizing antibody was added to the cultures, non-adherent CML cell proliferation was significantly inhibited. These observations indicate that CML cells can convert their morphology and function to adherent myofibroblasts, and produce a significant amount of cytokine to give a growth-promotion activity to CML cells. |
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Keywords: | Chronic myelogenous leukemia Myofibroblast CD133 Vascular endothelial growth factor Cancer stem cell |
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