Litopenaeus vannamei tumor necrosis factor receptor-associated factor 6 (TRAF6) responds to Vibrio alginolyticus and white spot syndrome virus (WSSV) infection and activates antimicrobial peptide genes |
| |
Authors: | Pei-Hui WangDing-Hui Wan Zhi-Hua GuXie-Xiong Deng Shao-Ping WengXiao-Qiang Yu Jian-Guo He |
| |
Institution: | a State Key Laboratory of Biocontrol/MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China b School of Marine Sciences, Sun Yat-sen University, 135 Xingang Road West, Guangzhou 510275, People's Republic of China c Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, MO 64110, USA |
| |
Abstract: | Tumor necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) is a key signaling adaptor protein not only for the TNFR superfamily but also for the Interleukin-1 receptor/Toll-like receptor (IL-1/TLR) superfamily. To investigate TRAF6 function in invertebrate innate immune responses, Litopenaeus vannamei TRAF6 (LvTRAF6) was identified and characterized. The full-length cDNA of LvTRAF6 is 2823 bp long, with an open reading frame (ORF) encoding a putative protein of 594 amino acids, including a RING-type Zinc finger, two TRAF-type Zinc fingers, a coiled-coil region, and a meprin and TRAF homology (MATH) domain. The overall amino acid sequence identity between LvTRAF6 and other known TRAF6s is 22.2-33.3%. Dual luciferase reporter assays in Drosophila S2 cells revealed that LvTRAF6 could activate the promoters of antimicrobial peptide genes (AMPs), including Drosophila Attacin A and Drosomycin, and shrimp Penaeidins. Real-time quantitative PCR (qPCR) indicated that LvTRAF6 was constitutively expressed in various tissues of L. vannamei. After Vibrio alginolyticus and white spot syndrome virus (WSSV) challenge, LvTRAF6 was down-regulated, though with different expression patterns in the intestine compared to other tissues. After WSSV challenge, LvTRAF6 was up-regulated 2.7- and 2.3-fold over the control at 3 h in gills and hepatopancreas, respectively. These results indicated that LvTRAF6 may play a crucial role in antibacterial and antiviral responses via regulation of AMP gene expression. |
| |
Keywords: | TRAF6 tumor necrosis factor receptor-associated factor 6 WSSV white spot syndrome virus TNFR tumor necrosis factor receptor IL-1 interleukin-1 receptor TLR Toll-like receptor ORF open reading frame MATH meprin and TRAF homology qPCR real-time quantitative PCR AMPs antimicrobial peptides PAMPs pathogen-associated molecular patterns IRAK4 IL-1 receptor-associated kinase-4 IKK IκB kinase MAPK mitogen-activated protein kinases JNK Jun-N-terminal kinases IRF IFN regulatory factor LB Luria broth RACE rapid amplification of cDNA end S2 Drosophila Schneider 2 FBS fetal bovine serum ALFs antilipopolysaccharide factors |
本文献已被 ScienceDirect 等数据库收录! |
|