YH-16对宫颈癌Hela细胞及其裸鼠皮下移植瘤作用研究

目的：初步探讨YH-16（重组内皮抑素）对宫颈癌Hela细胞及其荷瘤生长抑制作用及作用机制。方法：用MTY法检测YH-16对Hela细胞生长抑制作用；用透射电镜观察YH-16处理Hela细胞后的凋亡情况、流式细胞仪检测细胞凋亡率及细胞周期；将Hela细胞移植至裸鼠皮下成瘤，观察不同浓度YH-16（0mg／kg、0．4mg／kg、0．75mg／kg和1．5mg／kg）对荷瘤鼠皮下移植瘤生长的影响；TUNEL法观察肿瘤细胞的凋亡；免疫组化方法检测裸鼠移植瘤组织中肿瘤微血管密度（MVD）。结果：YH-16具有体外抑制Hela细胞增殖的作用（P〈0．01）；能诱导Hela细胞凋亡；YH-16能有效抑制裸鼠皮下移植瘤的生长（P〈0.05）；YH-1615mg／kg组的肿瘤MVD计数较对照组和其它治疗组明显降低（P〈0．05）。结论：YH-16具有抑制宫颈癌Hela细胞及其皮下移植瘤生长的作用。

Inhibition of YH 16 on the growth both Hela cells and Hela implanted tumor in nude mice

Objective：To evaluate the inhibitory effect of YH - 16 （rhEndostatin） on the growth of cervix cancer cell lines Hela and Hela implanted tumor in nude mice. Methods： The effect of endostatin on Hela cell proliferation was studied by means of MTT . The cell apoptosis was detected by transmission electron microscope and cell flow cytometry. The Hela cells were subcutaneously inoculated into nude mice. The therapeutic efficacy of different doses YH -16（0mg/kg,0. 4mg/kg, 0. 75mg/kg and 1.5mg/kg） on bearing cancer mice was detected. The cell apoptosis of implanted tumor was detected by TUNEL stanning. Results ： YH - 16 inhibited Hela cells proliferation （ P 〈 0.01 ） and induced cell apoptosis. YH - 16 significantly suppressed the growth of the implanted rumor in nude mice （P 〈 0.05）. The MVD in mice treated with YH- 16 15mg/kg was much lower than other groups（ P 〈0.05）. Conclusion： Endostatin had inhibitory effects on the growth of both Hela cell and implanted tumors in nude mice.