柴白妇乐颗粒质量标准的研究 |
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引用本文: | 罗文汇,胥爱丽,毕晓黎,孙冬梅. 柴白妇乐颗粒质量标准的研究[J]. 中国实用医药, 2009, 4(30): 17-18 |
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作者姓名: | 罗文汇 胥爱丽 毕晓黎 孙冬梅 |
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作者单位: | 广东省中医研究所,广州,510095 |
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摘 要: | 目的对柴白妇乐颗粒建立有效的质量控制方法。方法采用薄层色谱法对白芍、柴胡、败酱草、水蛭进行定性研究;采用高效液相色谱法,以甲醇-0.1%磷酸溶液(14:86)为流动相,检测波长为230nm,采用Phenomenex C18分析柱(4.6mm×250mm,5μm),测定方中芍药苷的含量。结果薄层色谱斑点清晰,空白对照无干扰。芍药苷260~1300ng范围内线性良好,平均回收率为97.52%,RSD为1.17%。结论所建立的方法简便、快速、准确,可有效地控制柴白妇乐颗粒的质量。
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关 键 词: | 柴白妇乐颗粒 质量标准 芍药苷 高效液相色谱法 |
Quality standard of Chaibaifule granules |
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Affiliation: | LUO Wen-hui, XU Ai-li, BI Xiao-li, et al( Guangdong Institute of Traditional Chinese Medicine, Guangzhou, Guangdong 510095) |
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Abstract: | Objective To establish a method for quality control of Chaibaifule granules. Methods Radix Paeoniae Alba, Radix Bupleuri, Herba Patriniae and Hirudo in Chaibaifule granules were identified by thin-layer chromatography. The Paeoniflorin was determined by HPLC. The codition of HPLC was Phenomenex C18(4.6 mm×250 mm,5 μm)column, methanol-0. 1% phosphoric acid( 14:86) as the mobile phase and detective wavelength at UV 230 nm. Results The chromatographic spots were clear,without interference and easy to recognize. The calibraction curve was linear within 260 - 1300 ng of paeoniflorin. The average recovery and RSD of Paeoniflorin were 97.52% and 1.17% ,respectively. Conclusion The method can be adopted for the quality control of Chaibaifule granules and the determination method of Peaoniflorin is simple, rapid and accurate. |
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Keywords: | Chaibaifule granules Quality specification Peaoniflorin HPLC |
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