柴白妇乐颗粒质量标准的研究

目的对柴白妇乐颗粒建立有效的质量控制方法。方法采用薄层色谱法对白芍、柴胡、败酱草、水蛭进行定性研究;采用高效液相色谱法,以甲醇-0.1%磷酸溶液（14：86）为流动相,检测波长为230nm,采用Phenomenex C18分析柱（4.6mm×250mm,5μm）,测定方中芍药苷的含量。结果薄层色谱斑点清晰,空白对照无干扰。芍药苷260～1300ng范围内线性良好,平均回收率为97.52%,RSD为1.17%。结论所建立的方法简便、快速、准确,可有效地控制柴白妇乐颗粒的质量。

Quality standard of Chaibaifule granules

Objective To establish a method for quality control of Chaibaifule granules. Methods Radix Paeoniae Alba, Radix Bupleuri, Herba Patriniae and Hirudo in Chaibaifule granules were identified by thin-layer chromatography. The Paeoniflorin was determined by HPLC. The codition of HPLC was Phenomenex C18（4.6 mm×250 mm,5 μm）column, methanol-0. 1% phosphoric acid（ 14：86） as the mobile phase and detective wavelength at UV 230 nm. Results The chromatographic spots were clear,without interference and easy to recognize. The calibraction curve was linear within 260 - 1300 ng of paeoniflorin. The average recovery and RSD of Paeoniflorin were 97.52% and 1.17% ,respectively. Conclusion The method can be adopted for the quality control of Chaibaifule granules and the determination method of Peaoniflorin is simple, rapid and accurate.