| 叶绿酸铜钠抗肿瘤作用的体外实验研究 |
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| 引用本文： | 丁小文,郑树,彭佳萍,吕庆华,黄建.叶绿酸铜钠抗肿瘤作用的体外实验研究[J].中国病理生理杂志,2004,20(9):1659-1663. |
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| 作者姓名： | 丁小文 郑树 彭佳萍 吕庆华 黄建 |
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| 作者单位： | 浙江大学肿瘤研究所, 浙江 杭州 310009 |
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| 摘 要： | 目的:研究叶绿酸铜钠(CHL)体外抑制HT29肠癌细胞的作用及机理。方法:①MTT法测半数抑制浓度(IC50)及生长曲线;②Wright-Giemsa染色、DNA电泳和流式细胞仪观察CHL对HT29细胞凋亡的诱导作用;③PCR-ELISA方法定量定性检测CHL对HT29细胞端粒酶的影响;④RT-PCR、Western blot方法检测环氧化酶2(COX-2)的表达。结果:①CHL能抑制HT29细胞生长且呈剂量依赖关系;②CHL能够使HT29细胞阻滞在G1期,但并不能诱导细胞凋亡;③CHL能够抑制HT29细胞端粒酶活性和COX-2的表达。结论:CHL能抑制HT29细胞生长,这种抑制作用同端粒酶活性抑制、COX-2表达下调及细胞G1期阻滞有关,但与细胞凋亡无关。
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| 关 键 词： | 叶绿素 端粒 末端转移酶 结直肠肿瘤 细胞凋亡 |
| 文章编号： | 1000-4718(2004)09-1659-05 |
| 收稿时间： | 2003-2-27 |
| 修稿时间： | 2003-4-21 |
Antitumor effect of chlorophyllin in vitro |
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| DING Xiao-wen,ZHENG Shu,PENG Jia-ping,LV Qing-hua,HUANG Jian.Antitumor effect of chlorophyllin in vitro[J].Chinese Journal of Pathophysiology,2004,20(9):1659-1663. |
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| Authors: | DING Xiao-wen ZHENG Shu PENG Jia-ping LV Qing-hua HUANG Jian |
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| Institution: | Cancer Institute, Zhejiang University, Hangzhou 310009, China |
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| Abstract: | AIM: To study the effect and mechanism of chlorophyllin (CHL) inhibiting HT29 cells. METHODS: IC 50 value and growth curve of HT29 cells were detected with MTT method. Apoptosis was detected with Wright-Giemsa staining, FCM and DNA electrophoresis. Telomerase was detected by PCR-ELISA, and protein and mRNA expression of COX-2 gene were detected through RT-PCR and Western blot. RESULTS: CHL inhibited the growth of HT29 in a dose-dependent manner. CHL blocked HT29 cells in G 1 phase but did not induce apoptosis. Different concentration of CHL inhibits the expression of telomerase and COX-2 in HT29 cells. CONCLUSION: CHL inhibits the growth of HT29 cells by inhibiting the expression of telomerase and COX-2 and blocking cells in G 1 phase. |
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| Keywords: | Chlorophyll Telomerase Colorectal neoplasms Apoptosis |
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