完全费氏佐剂预防非肥胖糖尿病鼠胰岛炎和糖尿病的效应

目的 探讨完全费氏佐剂（CFA）在预防非肥胖糖尿病（NOD）鼠胰岛炎和糖尿病机制中的作用。方法 将3周龄NOD雌鼠随机分入CFA组、磷酸盐缓冲液（PBS）对照组,分别给予后足板一次性注射CFA50μl、PBS50μl,于6周龄、12周龄、30周龄时观察胰岛β细胞凋亡、胰岛炎程度和糖尿病发病率。结果 6周、12周时CFA组胰岛炎积分、β细胞凋亡率均明显低于周期PBS组（P＜0.05,P＜0.01）,6周龄、12周龄胰岛炎积分与β细胞凋亡率存在正相关。到30周龄时,CFA组11只鼠只有1只发生糖尿病（1／11）,明显低于PBS组[11只中有9只（9／11）] ,P=0.001。结论 早期给予NOD鼠CFA可明显减轻其胰岛炎和糖尿病的发生,其机制与抑制β细胞凋亡有关。

The effects of complete Freund's adjuvant on prevention of pancrentitis and diabetes mellitus in non-obese diabetic mice

OBJECTIVE: To investigate the role of complete Freund's adjuvant (CFA) in preventing insulitis and diabetes in non-obese diabetic (NOD) mice. METHODS: Three-week-old female NOD mice were randomly divided into CFA group and phosphate buffered solution (PBS) group. The medications were injected once into the pad of hind foot with 50 microl CFA or 50 microl PBS respectively. Mice of the two groups were sacrificed at 6, 12 and 30 weeks of age respectively. Staining and counting the apoptotic beta cells in islets and evaluating insulitis severity and diabetes incidence were then carried out. The pancreatic sections were stained with hermatoxylin and eosin to score insulitis, and the apoptotic beta cells in islets were confirmed with the di-labeling technique of the TUNEL in situ end-labeling method combined with standard sensitive avidin-biotin complex (sABC) immunohistochemical method. RESULTS: The insulitis score of CFA-treated mice at 6 and 12 weeks of age was significantly lower than that of PBS-treated mice at the corresponding time respectively (P(6w) < 0.05, P(12w) < 0.05). The apoptotic beta cells were fewer in CFA-treated mice than that in PBS-treated mice at 6 and 12 weeks respectively (P(6w) < 0.01, P(12w) < 0.01). A positive correlation was found between the apoptotic beta cell rates and the insulitis scores at 6 and 12 weeks of age. The diabetes incidence in CFA-treated mice was lower than that in PBS-treated mice (P = 0.001). CONCLUSION: Early treatment with CFA can diminish insulitis and prevent the development of autoimmune diabetes in NOD female mice; this may result from inhibition of apoptotic beta cells.