Cytomegalovirus detection by biotinylated DNA probes |
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Authors: | S. M. Hussain Qadri Mohammed N. Al-Ahdal G. Yasmeen Khan |
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Affiliation: | (1) Pathology and Laboratory Medicine Department, King Faisal Specialist Hospital and Research Centre, 11211 Riyadh, Saudi Arabia;(2) Biological and Medical Research Department, King Faisal Specialist Hospital and Research Centre, 11211 Riyadh, Saudi Arabia;(3) Microbiology Division, King Saud University, Riyadh, Saudi Arabia;(4) St. Michael's Hospital, 75501 Texarkana, Arkansas, USA |
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Abstract: | Clinical specimens from 317 patients suspected of cytomeglovirus infection were examined by immunofluorescence (IF) using monoclonal antibodies and by a biotinylated DNA probe kit after cell culture isolation. Of the 317 samples, 68 were positive by culture isolation. Of these 67 were IF positive when the cytopathic effect (CPE) was 1+ or less, whereas 56 gave positive results with DNA probes when the CPE was 2+. A further 83 specimens were examined directly by immunoperoxidase histopathology (IHP), IF and the DNA probe kit: 26 of these were positive by IHP examination, 25 by IF and only 6 by DNA probes. The sensitivity of the DNA probe kit was not satisfactory when the clinical tissue specimens were directly examined. However, the sensitivity improved considerably to 82% if the specimens were propagated first in cell culture. The IF method detected the virus before and after cell culture isolation equally well (96%–98.5%). Compared to the IF method, the DNA probe kit is costly and requires more labor and time.This paper was presented in part at the 88th annual meeting of the American Society for Microbiology, Miami Beach, FL, USA, 1988 |
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