不同剂量花姜酮对大鼠重症急性胰腺炎的影响

目的:探讨花姜酮( Zerumbone)静脉给药对大鼠重症急性胰腺炎(SAP)的治疗作用。方法:雄性Wistar大鼠50只,随机分为5组:假手术组(SO组)、SAP模型组(SAP组)、5mg/kg花姜酮预处理组(5mg/kg花姜酮组)、10mg/kg花姜酮预处理组(10mg/kg花姜酮组)、20 mg/kg花姜酮预处理组(20mg/kg花姜酮组)。胆胰管逆行注射5%牛磺胆酸钠制备SAP模型。SO组、SAP组造模前30 min股静脉注射10%二甲基亚砜(DMSO)(2ml/kg),余三组注射等量10%DMSO溶解的等体积、不同浓度花姜酮。术后12h心脏取血检测各组血清淀粉酶(AMY),谷氨酸氨基转移酶(ALT),血肌酐(Cr)浓度变化,并观察胰腺组织病理学改变。结果:与SAP组比较,5 mg/kg花姜酮组AMY和胰腺病理评分没有改变,ALT和Cr有改善(P0.05);10mg/kg花姜酮组上述指标较SAP组显著降低(P0.05);20mg/kg花姜酮组AMY、Cr和胰腺组织病理学评分较SAP组显著降低(P0.05),但ALT与SAP组比较差异无统计学意义(P0.05)。结论:花姜酮治疗大鼠SAP,以10 mg/kg静脉注射比较安全、有效。

Dose-Response Relationship of Zerumbone in Rat with Severe Acute Pancreatitis

Objective:To explore the dose-response relationship of Zerumbone on severe acute pancreatitis(SAP) in rats.Method:Fifty male Wistar rats were randomly divided into five groups (n=10):sham operation group (SO group),SAP group,Zerumbone pretreatment groups at different dosage (5,10 and 20mg/kg).SAP model was induced by retrograde infusion of 5% sodium taurocholate (1 ml/kg) into the biliopancreatic duct.The rats of SO group and SAP group were injected with 10%DMSO (2 ml/kg) by femoral vein 30 min before the operation.Rats were killed at 12h after operation.Blood samples were obtained for detectingserum amylase,alanine aminotransferase and creatinine.Pancreatic tissue samples were stained with hematoxylin and eosin for pancreatic histopathological evaluation.Results:Compared with SAP group,5 mg/kg dosage failed to improve the levels of amylase and alanine aminotransferase,but attenuated severity of pancreatic injury(P<0.05).10 mg/kg dosage had the best effect on improving the levels of amylase,alanine aminotransferase and pathologic scores(P<0.05).The dosage of 20 mg/kg also improve the level of amylase and pathologic scores(P<0.05),but failed to improve the level of alanine aminotransferase (P>0.05).Conclusion:Zerumbone (10 mg/kg) administered intravenously was the optimal dosage for attenuating the severity of severe acute pancreatitis induced by retrograde infusion sodium taurocholate.