不同种质紫苏叶挥发性成分化学型研究

目的:分析不同种质紫苏叶挥发性成分的化学型,并探讨其种质、叶片颜色与化学型的关系.方法:采用气质联用技术(GC-MS),以P4峰为参照,绘制30批紫苏叶挥发性成分的指纹图谱,采用《中药色谱指纹图谱相似度评价系统(2004A版)》进行相似度评价,确定共有峰.采用同一GC-MS法测定紫苏叶挥发性成分;采用Qualitati...

Chemotype Study of Volatile Components from Perillae Folium of Different Germplasms

OBJECTIVE:To analyze the chemotypes of volatile components from Perillae Folium of different germplasms,and to investigate the relationship of germplasm and leaf color with chemotype.METHODS:The fingerprints of volatile components from 30 batches of Perillae Folium were prepared by GC-MS with P4 peak as reference.Similarity Evaluation System for TCM Chromatographic Fingerprint(2004A edition)was applied to evaluate the similarity and confirm common peaks.The volatile components of Perillae Folium were determined by the same GC-MS method.Qualitative Navigator(B.08.00)software was used to analyze and compare with NIST 17.0 standard mass spectrum database.The compounds corresponding to the peak were analyzed;clustering analysis was carried out with Origin 2018 software.RESULTS:There were 13 common peaks in the fingerprints of volatile components from 30 batches of Perillae Folium.The similarities were 0.13-1.00.Totally 54 components were identified from 30 batches of Perillae Folium of different germplasm.Cluster analysis showed that 30 batches of Perillae Folium samples could be clustered into three categories;among them,SCY-1,YNT-9,YNX-17,YN-28 were clustered into one category,with phenylpropanoid-elemicin(PP-e as)the main volatile component,being PP-e type;GS-4,GS-7,GS-11,GS-19,HBA-14,HBA-20,GZZ-8,LN-39,GSL-27,GSQ-32,GSQ-33,GST-31,YNW-12,LN-38 were clustered into one category,and the content of perilla ketone(PK)in them was the highest except for LN-38,being PK type[the content of phenylpropanoid-apiol(PP-a)in LN-38 was higher than that of perilla ketone,being PP-a type];HBS-2,HBS-3,HBS-6,HBS-15,HBS-16,HBS-24,HBS-25,GX-26,SXS-30,SCC-36,RB-37,SC-29 were clustered into one category,and the content of perillaldehyde(PA)was the highest,being PA type.The color characteristics of Perillae Folium of different germplasm showed that Perilla frutescens(L.)Britt.var.frutescens with green leaves on both sides was PK type,while P.frutescens(L.)Britt.var.arguta with purple leaves on one or both sides was PA type,and P.frutescens(L.)Britt var.auriculato-dentata C.Y.Wu et Hsuan ex H.W.Li was PP-e type.CONCLUSIONS:The chemotype of volatile components in Perillae Folium have a certain corresponding relationship with their leaf colors.Most of P.frutescens(L.)Britt.var.arguta with purple leaves on one side or both sides are PA type.P.frutescens(L.)Britt.var.acuta(Thunb.)Kudo,P.frutescens(L.)Britt var.auriculato-dentata C.Y.Wu et Hsuan ex H.W.Li and P.frutescens(L.)Britt.var.frutescens with green leaves on both sides do not belong to PA type,among which P.frutescens(L.)Britt var.frutescens is PK type,while P.frutescens(L.)Britt var.auriculato-dentata C.Y.Wu et Hsuan ex H.W.Li is mostly PP-e type.