石蜡包埋表皮组织中β-连环蛋白基因扩增技术的建立

目的：建立从石蜡包埋表皮组织中提取DNA，进行β-连环蛋白基因3号外显子聚合酶链反应(PCR)扩增的方法。方法：从14例不同重量、不同年代石蜡包埋皮肤鲍温氏病病变组织中提取DNA，再进行β-连环蛋白基因3号外显子PCR扩增。结果：5mg组织提取DNA的PCR扩增未获成功，而用10mg和20mg组织提取的DNA可成功扩增；已包埋10年以上的5例标本仅2例成功扩增，而9例10年以内的标本全部成功。结论：10mg及10mg以上且10年以内的石蜡包埋表皮组织可用于目标基因PCR扩增。

Amplification of beta-catenin gene in the paraffin-embedded epidermal tissue]

OBJECTIVE: To establish a method of extracting DNA from the paraffin-embedded epidermal tissue and examine the amplification of beta-catenin gene by polymerase chain reaction (PCR) analysis. METHODS: Exon 3 of the beta-catenin gene was amplified by PCR in 14 paraffin-embedded Bowen's disease (BD) of various weight and years. RESULTS: The amplification of exon 3 of beta-catenin gene was successful in all the 10 mg and 20 mg samples, but not in the 5 mg samples. The amplification of exon 3 of beta-catenin gene was successful in 9 samples embedded within 10 years, and in only 2 of the 5 samples embedded more than 10 years. CONCLUSION: Epidermal tissue whose weight is 10 mg or more within 10 years of embedding can be used in the amplification of purpose gene by PCR.