鼻咽泡状核细胞癌超微结构的研究

目的观察泡状核细胞癌的超微结构及免疫组化特点,探讨其病因、组织起源与分型等问题。方法收集18例鼻咽泡状核细胞癌的活检标本,应用常规HE染色及免疫组化染色,其中10例于活检同时进行常规电镜制片。结果电镜下1例癌细胞核内见有直径约45nm的电子致密颗粒,考虑为乳头多瘤空泡病毒。癌细胞胞质泡状系统占优势,而张力微丝少见,桥粒发育不良,应将其列为柱状细胞癌,癌组织可能起源于鼻咽纤柱上皮的中间细胞或基底细胞。免疫组化CK-HMW染色,大多数呈阳性反应。结论电镜与免疫组化观察有助于泡状核细胞癌的病理鉴别诊断。

Ultrastructural Research about the Nasopharyngeal Vesicular Nucleus Cell Carcinoma

Objective Observe the ultrastructure of vesicular nucleus cells and the characters of immunohistochemical, and study the problems about its pathogeny and tissue origins and classification. Methods Collect eighteen biopsy samples of nasopharyngeal vesicular nucleus and stain them with routine HE and immunohistochemistry. Ten samples of them were done the electron microscope observation. Results There are dense granules discovered from one of ten samples under the electron microscope which have a diameter of about 45nm. They are considered as papovaviridaes. The vesicular system in the cancer cell cytoplast predominate and tonomicrofilament see little of. Desmosomes are hypogenesis. This sample should be classfied as vesicular cell carcinoma according above. The carinoma tissue probably stemed from the intermediate cell or basal cell of nasopharyngeal ciliated columnar epithelium. Most samples presented positive reaction in the immunohistochemical CK-HMW stain. Conclusion Electron microscope and immunohistochemical observation are contributed to the pathology classification and diagnosis on vesicular nucleus cell carcinoma.