川芎嗪对骨髓移植小鼠骨髓造血的影响

目的：探讨川芎嗪对同基因骨髓移植（bone marrow transplantation,BMT)小鼠BMT后骨髓造血的影响。方法：建立典型同基因BMT小鼠模型，随机分成两组：骨髓移植（BMT）组和BMT加川芎嗪处理组（川芎嗪组），另设正常组（未做任何处理）。BMT组胃饲生理盐水，每次0.2ml/kw，每天2次。川芎嗪组胃饲磷酸川芎嗪注射液，每次0.2ml/只，每天2次。分别于BMT后第1、7、14天观察外周血细胞，骨髓单个核细胞（BMMNC），采用免疫组化SABC－AP法测定骨髓组织切片中硫酸肝素（HS）表达水平；基质细胞来源因子－1（stromal derived factor,SDF-1)的表达水平；CXC趋化因子受体4（CXCR4）表达水平；基质细胞来源因子－1（stromal derived factor,SDF-1)的表达水平；CXC趋化因子受体4（CXCR4）表达水平。结果：川芎嗪组第7、14天外周血白细胞、红细胞、血小板、BMMNC及CXCR4、HS、SDF－1表达水平均显著高于BMT组（P＜0．05或P＜0．01）。结论：川芎嗪能够在骨髓移植造血重建早期促进骨髓造血。

Effect of Ligustrazine on Bone Marrow Hematopoiesis in Mice after Bone Marrow Transplantation

Objective: To investigate the effect of ligustrazine (LT) on hematopoiesis in mice after bone marrow isotransplantation (iso BMT). Methods: The typical model of iso BMT was established and the model mice were randomly divided into two groups, the LT group treated with LT injection 0.2 ml and the control group treated with normal saline 0.2 ml, twice a day by gastrogavage. The following parameters were observed in the day 1, 7 and 14: peripheral blood cells, bone marrow mono nuclear cells (BMMNC), heparin sulfate (HS) expression in bone marrow section by immunohistochemical SABC AP method, stromal cell derived factor 1 (SDF 1) expression and CXC chemotaxis factor receptor 4 (CXCR4) expression. Results: The levels of peripheral WBC, platelet, BMMNC, CXCR4, HS, SDF 1 at the day 7 and 14 in the LT group were all higher significantly than those in the control group (P<0 05 or P<0 01). Conclusion: LT could improve the bone marrow hematopoiesis in the early hematopoietic re establishing stage after BMT.