| 大鼠脊髓缺血再灌注损伤后神经细胞凋亡及Caspase-1、Caspase-3的表达 |
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| 引用本文： | 韩亚新,王岩峰,屠冠军.大鼠脊髓缺血再灌注损伤后神经细胞凋亡及Caspase-1、Caspase-3的表达[J].解剖科学进展,2011,17(6):559-561,565. |
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| 作者姓名： | 韩亚新 王岩峰 屠冠军 |
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| 作者单位： | 中国医科大学附属第一医院骨科,辽宁沈阳,110001 |
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| 基金项目： | 辽宁省教育厅科研基金资助项目(No.L2010687) |
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| 摘 要： | 目的观察大鼠脊髓缺血再灌注损伤后神经细胞凋亡及Caspase-1、Caspase-3的表达。方法 48只成年健康Wistar大鼠,采用Naslund腹主动脉阻断法制作脊髓缺血再灌注损伤模型。随机分为空白对照组(n=6)、假手术组(n=6)和损伤组(n=36),观察脊髓缺血再灌注损伤后,脊髓的病理变化、细胞凋亡及Caspase-1、Caspase-3的表达变化的规律。结果观察脊髓缺血再灌注损伤后Caspase-1阳性细胞在损伤后12h表达明显,1d损伤区域及周边出现表达高峰。Caspase-3阳性细胞在损伤后12h表达明显,2d损伤区域及周边出现表达高峰。TUNEL阳性细胞也在脊髓损伤后12h表达明显,2d出现表达高峰。空白对照组、假手术组:Caspase-1、Caspase-3免疫组化少有表达,少见TUNEL阳性细胞。结论脊髓缺血再灌注损伤后存在神经细胞凋亡,Caspase-1、Caspase-3均参与损伤的调节。
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| 关 键 词： | 脊髓缺血再灌注 细胞凋亡 Caspase-1 Caspase-3 大鼠 |
Cellular apoptosis and Caspase-1 and Caspase-3 expressions following rat spinal cord ischemia/reperfusion injury |
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| HAN Ya-xin,WANG Yan-feng,TU Guan-jun.Cellular apoptosis and Caspase-1 and Caspase-3 expressions following rat spinal cord ischemia/reperfusion injury[J].Progress of Anatomical Sciences,2011,17(6):559-561,565. |
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| Authors: | HAN Ya-xin WANG Yan-feng TU Guan-jun |
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| Institution: | HAN Ya-xin,WANG Yan-feng,TU Guan-jun*(Department of Orthopaedics,The first affiliated Hospital of China Medical University,Shenyang 110001 China) |
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| Abstract: | Objective To observe the neural cell apoptosis and the changes of Caspase-1 and Caspase-3 expressions after spinal cord ischemia/reperfusion injury.Method The spinal ischemia-reperfusion lesions models of healthy adult Wistar rats(48)were created by using of Naslund abdominal aorta occlusion.The animals were divided into normal group(n=6),sham operation group(n=6)and injuried group(n=36).The apoptosis of neural cells,the expressions of Caspase-1 and Caspase-3 were studied by TUNEL and immunochemistry method... |
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| Keywords: | spinal cord injury apoptosis caspase-1 caspase-3 rat |
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