桔梗总皂苷对人肺腺癌细胞系A549移植裸鼠瘤生长和STAT2的影响

Effects of total platycodins on the growth of transplantation tumor of human lung adenocarcinoma A549 and STAT2 expression in nude mice

Objective To investigate the inhibition effects of total platycodins on thetransplantation tumor growth of human lung adenocarcinoma cell line A549 and STAT2 expression in nude mice. Methods The BALB/c nude mice bearing human A549 lung cancer were randomly divided into model group, cyclophosphamide group, platycodins group 1 （5 mg/kg-d）, platycodins group 2 （10 mg/kg-d）, platycodins group 3 （20 mg/kg .d） and platycodins group 4 （25 mg/kg .d）. They were treated and observed for 30 days, and tumor volume and tumor mass were tested, morphology and the tumor tissues were observed by H-E staining, STAT2 expression was detected by immunohistochemistry. Results There were significant differences between model group and each total platycodins group in the growth of the transplantation ttmaor of human lung adenocarcinoma cell line A549 in nude mice（P〈0.05）. There were better inhibition effects on transplantation tumor sizes and tumor inhibition rates in all total platycodins groups. The transplantation tumor sizes in platycodins group 4 was significantly lower than those of model group and other platycodins groups（P〈0.05）; transplantation tumor sizes in all total platycodins groups were greater than that of cyclophosphamide group （P〈0.05）; STAT2 expression in platycodins groups were reduced gradually with the increasing of total platycodins （P〈0.05） and showed concentration-dependent effects. Conclusion Platyeodins have good inhibition effects on A549 lung tumor, which maybe related with the down-regulation of STAT2 expression.