应用组织微阵列和原位杂交检测卵巢上皮性肿瘤中HIF-1α mRNA的表达

目的　探讨缺氧诱导因子 1α(HIF 1α)在卵巢上皮性肿瘤中的表达及意义。方法　采用组织微阵列 (TMA)新技术和原位杂交方法 ,回顾性研究 2 95例卵巢上皮性肿瘤中HIF 1αmRNA的表达情况 ,并以 13例正常卵巢组织作对照。结果　正常卵巢、卵巢上皮性良性肿瘤、交界性肿瘤和卵巢癌组织的HIF 1αmRNA表达阳性率分别为 0、13.2 %、4 2 .1%和 81.9%。交界性肿瘤和卵巢癌组织中的HIF 1αmRNA表达显著高于正常卵巢和良性卵巢上皮性肿瘤 (P <0 .0 0 1) ,卵巢癌显著高于交界性肿瘤 (P <0 .0 0 1)。卵巢癌组织中HIF 1αmRNA的表达与临床病理分期和病理类型无相关性 (P >0 .0 5 )。但在不同组织学分级 (即高、中、低分化 )的卵巢癌组织中 ,HIF 1α表达率分别为 6 0 .4 %、81.2 %和 96 .4 % ,与组织学分化程度呈负相关 (P <0 .0 0 1)。结论　HIF 1α在卵巢癌的发生、发展过程中可能起着重要作用。组织微阵列技术适用于大样本量组织的检测 ,是一种高效、快速、可比性强的分子生物学研究方法。

Tissue microarray and in situ hybridization study on hypoxia inducible factor 1alpha mRNA expression in epithelial ovarian tumor

OBJECTIVE: To study the expression and significance of hypoxia inducible factor 1alpha (HIF-1alpha) in epithelial ovarian tumors. METHODS: The expression of HIF-1alpha mRNA in 295 patients with epithelial ovarian tumor was analyzed retrospectively by high-throughput tissue microarray and in situ hybridization, which was compared with 13 normal ovarian tissue samples. RESULTS: The expression rates of HIF-1alpha mRNA were 0, 13.2%, 42.1% and 81.9% in normal ovarian tissue, benign, borderline and malignant ovarian tumors. Expression rate of HIF-1alpha mRNA in borderline and invasive tumor was significantly higher than those in normal ovarian tissue and benign tumor (P < 0.001). Statistical analysis revealed that the expression of HIF-1alpha mRNA was not related to FIGO stages or histological subtypes. Close negative relation was observed between the expression of HIF-1alpha mRNA and tumor histological differentiation (P < 0.001). CONCLUSION: The overexpression of HIF-1alpha may play an important role in oncogenesis of epithelial ovarian tumor. Tissue microarray is an efficient technique of molecular biology.