Effects of epitopes combination and adjuvants on immune responses to anti-Alzheimer disease DNA vaccines in mice

Alzheimer disease （AD） is a neurodegenerative disorder characterized by neuropathological hallmarks including deposits of the β-amyloid peptide （AssP）. Studies have shown that immunization with Aβ42 peptide reduces both the spatial memory impairments and Alzheimer disease-like neuropathologic changes in Alzheimer disease transgenic mice, but can cause side effect of a cell-mediated autoimmune meningoencephalitis. Recently, some studies showed that DNA vaccination could be used to generate an antibody response to Aβ without the adverse cell-mediated immune effect. In the current study, we generate four DNA vaccine plasmids （pV-GE1, pV-GE2, pV-GE3, and pV-GE4） against Alzheimer disease by separately fusing Aβ epitope sequences （coding for EFGH, DAEFGH, EFGH＋EFGH, and EFGH＋DAEFGH） with IgG heavy chain coding region of mouse. Meanwhile, the full-length gene Aβ encoding plasmid （pV-Aβ）, empty vector （pVAX） and synthetic AssP were also included as control. The sera of BALB/c mice immunized via intramuscular with plasmids and peptide were tested by indirect

Effects of epitopes combination and adjuvants on immune responses to anti-Alzheimer disease DNA vaccines in mice