| 大鼠窦状卵泡颗粒细胞中磷酸化蛋白激酶B表达与凋亡的关系 |
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| 引用本文： | 杨晓葵,宋蕊,贾婵维,王树玉. 大鼠窦状卵泡颗粒细胞中磷酸化蛋白激酶B表达与凋亡的关系[J]. 中国优生与遗传杂志, 2010, 0(8): 14-15,F0002 |
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| 作者姓名： | 杨晓葵 宋蕊 贾婵维 王树玉 |
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| 作者单位： | 首都医科大学附属北京妇产医院生殖医学科,北京100026 |
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| 基金项目： | 国家人事部留学回国人员科技活动基金资助; 香港大学李嘉诚医学院郑裕彤博士奖学金（2008-2009年度）资助 |
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| 摘 要： | 目的探讨大鼠窦状卵泡中磷酸化蛋白激酶B(PKB,又称Akt)的表达与卵泡颗粒细胞凋亡之间的关系。方法应用免疫组织化学法和Western blot法检测磷酸化Akt(phosphorylation-Akt,p-Akt)在大鼠不同发育阶段卵泡颗粒细胞中的表达,应用TUNEL法检测成年大鼠卵泡颗粒细胞的凋亡情况。结果在大鼠窦前及窦状卵泡的颗粒细胞中,p-Akt表达高的颗粒细胞凋亡程度较轻,而p-Akt低表达的颗粒细胞凋亡现象较明显。结论 p-Akt的高表达在一定程度上与颗粒细胞凋亡受抑制有关,并有可能参与了窦状卵泡阶段卵泡的闭锁的选择。
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| 关 键 词： | 颗粒细胞 蛋白激酶B/Akt 磷酸化 凋亡 |
The effect of akt phosphorylation on apoptosis in granulosa cells of rat antral follicle |
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| YANG Xiao-kui,SONG Rui,JIA Chan-wei,WANG Shu-yu. The effect of akt phosphorylation on apoptosis in granulosa cells of rat antral follicle[J]. Chinese Journal of Birth Health & Heredity, 2010, 0(8): 14-15,F0002 |
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| Authors: | YANG Xiao-kui SONG Rui JIA Chan-wei WANG Shu-yu |
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| Affiliation: | .(Department of Human Reproductive Medicine,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing,100026) |
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| Abstract: | Objective：To explore the relationship between Protein kinase B（PKB/Akt） phosphorylation and apoptosis in granulosa cells of rat antral follicle.Methods：The expression of phosphorylated-Akt was determined in rat granulosa cells of antral follicles by immunohistocheistry and Western blot,as well as the apoptotic granulosa cells were detected by TUNEL（terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling）.Results：Phosphorylated-Akt was determined in preantral and antral granulosa cells.The more phosphorylated-Akt expression was associated with the less apoptotis in the rat granulosa cells of antral follicles.Conclusion：Phosphorylated-Akt inhibits apoptosis in rat granulosa cells and it may contribute to the atresia and recruitment of follicles. |
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| Keywords: | Granulosa cell PKB/Akt Phosphorylation Apoptosis |
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