提前复苏分裂速率较慢胚胎对妊娠率的影响

目的探讨冻融胚胎移植(FET)周期中,提前一日复苏冷冻前分裂速率较慢的胚胎对妊娠结局的影响。方法回顾性分析哈尔滨医科大学附属第一医院生殖中心2006年3月至2009年9月561例FET周期中,移植冷冻前分裂速率较慢胚胎的周期共88个。其中,33个周期为移植当日复苏胚胎,培养1-4h后移植。55个周期为移植前一日复苏胚胎,培养18-24h移植。比较两组胚胎移植后的妊娠率和种植率。结果移植当日复苏组与移植前一日复苏组妊娠率(9.1%,32.7%)与种植率(4.2%,15.5%)均有显著性差异(P﹤0.05);而患者平均年龄、移植前平均内膜厚度、胚胎复苏存活率及100%卵裂球存活率均无显著性差异(P﹥0.05)。结论对冷冻前分裂速率较慢胚胎提前一日复苏,可提高临床妊娠率和种植率。

Impact of thawing frozen slowly-cleaved embryos in advance on the pregnancy rate

Objective：To study the impact of thawing frozen slowly-cleaved embryos in advance on the pregnancy outcome in frozen-thawed embryo transfer（FET） cycles.Methods：In this retrospective study,a total of 88 cycles transferred with frozen slowly-cleaved embryos,which were from 561 FET cycles between March 2006 and September 2009,were divided into two groups（thawing-at-normal-time group in which embryos were thawed on transfer day and cultured 1-4h before transfer,thawing-in-advance group in which embryos were thawed one day in advance and cultured 18-24h before transfer） according to the thawing time,the pregnancy rates and implantation rates were compared in these two groups.Results：The pregnancy rate and implantation rate of thawing-at-normal-time group were significantly lower than those of thawing-in-advance group,（9.1% vs.32.7%,4.2% vs.15.5% respectively;P 0.05）.There were no significantly differences between the two groups in the mean age of patients,the endometrial thickness and the ratios of survived embryos or embryos survived intactly after thawing（P 0.05）.Conclusions：It could improve pregnancy rate and implantation rate significantly if the frozen slowly-cleaved embryos thawed in one day advance.