烧伤后不同类型胶原在增生性瘢痕发生中的作用研究

目的观察烧伤后瘢痕形成过程中Ⅰ、Ⅲ型胶原纤维的动态变化。方法采用苦味酸天狼猩红染色法对不同时期的瘢痕组织进行Ⅰ、Ⅲ型胶原纤维观察，RIA法对瘢痕组织中的Ⅰ、Ⅲ型前胶原进行检测。结果偏光观察正常皮肤中以Ⅲ型纤维为主，1月以后Ⅰ型纤维明显增加，1年以后的瘢痕组织中几乎全部为粗大的Ⅰ型纤维，极少Ⅲ型纤维。放免结果Ⅰ/Ⅲ型前胶原比例逐渐增加，但Ⅲ型前胶原的含量仍然较高；晚期瘢痕中Ⅰ、Ⅲ型前胶原含量均显著降低。结论烧伤后瘢痕形成过程中Ⅰ型胶原逐渐增加，Ⅲ型胶原逐渐减少，后期几乎完全为Ⅰ型胶原纤维取代，前胶原的变化与胶原纤维的变化不吻合，不能反映胶原纤维的实际情况。

The function of different collogens in hypertrophic scars

Objective The aim of this study was toinvestigate the dynamic changes of collagen Ⅰ and Ⅲ in the formation of hypertrophic scar afterbum. Method The sirius red and polarizationmicroscopy was used to detected the collagen Ⅰ and Ⅲ fibers in scar tissues in vary periods. Radial immunology assay methods were used to evaluatethe contents of procollagen Ⅰ and Ⅲ in vary periodsin HS tissue. Result Collagen Ⅲ fiber is the main collagen in normalskin. There were no collagen fibers been found in granulation tissue. Collagen Ⅰ increased significantly in scar tissue 1 month after burn, increased much more in 3 months than in 1 month. The collagen were nearly thick collagen Iin HS tissue 1.5 year afterburn and only a little collagen Ⅲ were found. The RIA result showed that the ratio of procollagen Ⅰ/Ⅲ were increased gradually but the contents of collagen Ⅲ were much higher than normal skin. Conclusion In the process of HS forming after burn collagen Ⅰ increase and collagen Ⅲ decrease gradually and at last the collagens in HS were almost collagen Ⅰ. The zchanges of procollagcn can not reflect the changes of collagen fibers.