母—胎免疫识别低下型反复自然流产的供者及配偶白细胞免疫 …

比较供者及配偶白细胞免疫疗法治疗母－胎免疫识别低下型反复自然流产的临床疗效。方法将４０例封闭抗体缺乏的ＲＳＡ患者随机分成两组,分别采用供者白细胞与配偶白细胞行免疫治疗,封闭抗体及抗其独物型抗体分析采用单向混合淋巴反应封闭试验,单向混合淋巴细胞反应封闭抑制试验,细胞毒试验及封闭抗体的流式细胞分析。结果经供者或配偶白细胞后,封闭抗体扩抗其独特体呈明显升高（Ｐ〈０．０５,Ｐ〈０．０１）。再次妊娠成功或配

Comparative study of the third party and paternal leukocyte immunization in recurrent spontaneous abortion of lowered maternal-fetal immuno-recognition

OBJECTIVE: To compare curative effect of leukocyte immunization to recurrent spontaneous aborters(RSA) with lowered maternal-fetal immuno-recognition between transfusions of the third party leukocyte-rich erythrocyte concentrates and injections of paternal leukocytes. METHODS: Forty RSA patients with lack of blocking antibodies were randomly divided into two groups. Blocking antibodies were analysed by one-way mixed lymphocyte reaction blocking test and complement-dependent cytotoxic test, anti-idiotypic antibodies to blocking antibodies by one-way mixed lymphocyte reaction blocking inhibiting test, and flow cytometry evaluation of blocking antibodies were used to investigate effect of blocking antibodies on the CD antigens of T cells. The immunization was achieved by transfusions of the third party leukocytes or injections of the paternal leukocytes for 3 times or more at an interval of 4 weeks. RESULTS: It was found in dynamical analyses that levels of the blocking antibodies and their anti-idiotypic antibodies increased significantly after the aborters had been immunized. The successful pregnancy rate was 86.2% following immunization, and body weight of the infants was 3,300 +/- 116 g(x +/- s), with no significant difference to 3,279.0 +/- 113.8 g of the infants from normal pregnancy. According to dynamics of blocking antibodies and their anti-idiotypic antibodies, the paternal leukocyte immunotherapy appeared superior to the third party one, but the successful pregnancy rate and the body weight of infants were with not different between the two immunotherapy groups. CONCLUSION: The third party and paternal leukocyte immunization could effectively stimulate the aborters of lowered maternal-fetal immuno-recognition to produce blocking antibodies and their anti-idiotypic antibodies, leading to successful pregnancy. The immunotherapy does not apparently influence the body weight of the delivered infants.