| 不同证候H22荷瘤小鼠胸腺一致上下调高表达的基因 |
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| 引用本文： | 丁善萍,方肇勤,卢文丽,潘志强,梁超,刘小美,侯俐,高必峰. 不同证候H22荷瘤小鼠胸腺一致上下调高表达的基因[J]. 辽宁中医药大学学报, 2010, 0(3) |
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| 作者姓名： | 丁善萍 方肇勤 卢文丽 潘志强 梁超 刘小美 侯俐 高必峰 |
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| 作者单位： | 上海中医药大学;美国科罗拉多大学; |
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| 基金项目： | 上海市科委国际合作项目(064307052);;上海市教委学科建设项目(2006) |
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| 摘 要： | 目的:观察常见证候荷瘤小鼠胸腺中一致上调和下调的高表达基因。方法:采用荷瘤小鼠及其标准化四诊及辨证方法,以及GeneChip Mouse Exon1.0ST Array等技术,检测H22荷瘤小鼠在早期邪毒壅盛证、气虚证,中期阳虚证,中晚期阴虚证等4个证候胸腺中一致上调或下调者,重点关注表达量高的基因。结果:肿瘤发生后,胸腺中有34个基因表达发生显著改变。其中一致上调者20个,一致下调9个。结论:(1)Tpp2、per2基因在肿瘤发生的早期表达几乎关闭,与肿瘤发生的早期关系密切。(2)20个高表达基因,如Hey2、MMP-19等,可能在中晚期胸腺退化阶段起到特殊的作用。(3)推测这些一致上调和下调的基因与肿瘤发生及其不同阶段的关系更为密切,而非证候。
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| 关 键 词： | 小鼠 肿瘤 胸腺 证候 基因 基因芯片 |
Coincident Up-regulated or Down-regulated High Expressed Genes in Thymus of H22 Tumor-Bearing Mice of Different Syndromes |
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| DING Shan-ping,FANG Zhao-qin,LU Wen-li,PAN Zhi-qiang,LIANG Chao,LIU Xiao-mei,HOU Li,GAO Bi-feng. Coincident Up-regulated or Down-regulated High Expressed Genes in Thymus of H22 Tumor-Bearing Mice of Different Syndromes[J]. Journal of Liaoning University of Traditional Chinese Medicine, 2010, 0(3) |
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| Authors: | DING Shan-ping FANG Zhao-qin LU Wen-li PAN Zhi-qiang LIANG Chao LIU Xiao-mei HOU Li GAO Bi-feng |
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| Affiliation: | 1.Shanghai University of Traditional Chinese Medicine/a>;Shanghai 201203/a>;China/a>;2.Medical College of America Coloroda State University/a>;Denver 80262/a>;USA |
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| Abstract: | Objective: To investigate the characteristics of coincident up- or down-regulated high expressed genes in thymus of H22 tumor-bearing mice of different syndromes.Methods: The thymus gene expression in H22 tumor-bearing mice of pathogenic toxin-exuberance syndrome and qi deficiency syndrome on early stage, yang-qi deficiency syndrome on intermediate stage, qi-yin-yang deficiency syndrome on advanced stage were detected with quantitative four diagnosis and syndrome differentiation methods and GeneChip Mouse E... |
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| Keywords: | mice tumour thymus symptoms and signs gene genechip |
| 本文献已被 CNKI 等数据库收录！ |
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