| 人碱性成纤维细胞生长因子基因真核表达载体的构建 |
| |
| 引用本文： | 吴宏,张绍昆,王桂云,张晓光.人碱性成纤维细胞生长因子基因真核表达载体的构建[J].吉林大学学报(医学版),2002,28(4):352-354. |
| |
| 作者姓名： | 吴宏 张绍昆 王桂云 张晓光 |
| |
| 作者单位： | 1. 吉林大学第二医院眼科,吉林,长春,130041;2. 吉林大学第一医院 |
| |
| 摘 要： | 目的:构建人碱性成纤维细胞生长因子bFGF基因真核表达载体,探讨bFGF基因转移治疗视网膜病变的方法.方法:将bFGF和GFP基因克隆到真核表达载体pcDNA3中.结果:酶切及琼脂糖电泳分析 pcFG中含有bFGF和GFP基因.结论:成功的构建了含有绿色荧光蛋白的人碱性成纤维细胞生长因子基因的真核表达载体.
|
| 关 键 词： | 绿色荧光蛋白 碱性成纤维细胞生长因子 基因 真核表达载体 |
| 文章编号： | 1671-587X(2002)04-0352-03 |
| 修稿时间： | 2001年11月29日 |
Construction of eukaryotic expression vector containing human basic fibroblast growth factor gene |
| |
| WU Hong ,ZHANG Shao kun ,WANG Gui yun ,ZHANG Xiao guang.Construction of eukaryotic expression vector containing human basic fibroblast growth factor gene[J].Journal of Jilin University: Med Ed,2002,28(4):352-354. |
| |
| Authors: | WU Hong ZHANG Shao kun WANG Gui yun ZHANG Xiao guang |
| |
| Institution: | WU Hong 1,ZHANG Shao kun 2,WANG Gui yun 1,ZHANG Xiao guang 1 |
| |
| Abstract: | Objective: To construct eukaryotic expression plasmid of human basic fibroblast growth factor gene (hbFGF) and green fluorescent protein (GFP) for further study on bFGF gene therapy of retinitis pigmentosa. Methods: The hbFGF and GFP genes were inserted into the eukaryotic expression plasmid pcDNA3. Results: The pcFG plasmid was confirmed to containe correct nucleotide sequence for hbFGF and GFP gene by restriction endounclease digestion and agarose gel electrophoresis. Conclusion: A eukaryotic expression plasmid containing hbFGF and GFP gene is successfully constructed in the study. |
| |
| Keywords: | green fluorescent protein basic fibroblast growth factor gene eukaryotic expression plasmid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
