淫羊霍对环磷酰胺致大鼠附睾结构和功能改变的拮抗作用

目的:探讨淫羊藿对化疗药物引起雄性大鼠附睾损伤的逆转作用。方法:60d的雄性SD大鼠50只,随机分为对照组、化疗1组、化疗2组和中药1组、中药2组,每组10只,化疗组接受腹腔内注射环磷酰胺30mg/(kg.d),中药组除环磷酰胺外,同时给予淫羊霍配制液灌胃30mg/(100g.d)。化疗1组、中药1组和化疗2组、中药2组分别于用药10、20d后观察附睾上皮细胞超微结构的改变。结果:①电镜显示化疗1组、2组大鼠附睾上皮细胞主要损伤为细胞水肿,线粒体肿胀,部分空泡化,细胞核呈瘤细胞样改变,部分核染色质凝集边移,趋于凋亡。中药1组上皮细胞超微结构与对照组基本相似,中药2组上皮细胞仅有轻度损伤,除线粒体嵴结构不清,胞浆内有少量空泡,部分核膜内陷或外突外,其余结构类同对照组。②化疗2组与对照组比较精子数量(10.63±3.82)×106/mlvs(15.59±4.01)×106/ml,P<0.01]、精子存活率(60.03±7.54)%vs(76.71±10.11)%,P<0.01]和唾液酸含量(13.62±7.81)mg/gprotvs(19.38±9.34)mg/gprot,P<0.05]显著降低;淫羊藿能明显升高精子存活率,化疗2组精子存活率显著低于中药2组(60.03±7.54)%vs(69.90±12.58)%,P<0.05]。结论:淫羊藿可减轻化疗造成的附睾损伤,对生殖功能具有保护作用。

Epimedium Alleviates Chemotherapy-induced Damage to the Ultrastructure and Function of Rat Epididymides

OBJECTIVE: To investigate the protective action of Epimedium against chemotherapy-induced damage to rat epididymides. METHODS: Fifty 60-day-old male rats were divided into a control, a model and a treatment group. Procarbazine was injected into the abdominal cavity of the model rats at the dose of 30 mg/(kg x d). In addition to procarbazine, Epimedium was given intragastrically to the treatment group. The changes in the ultrastructure of the epididymis were observed after 10 and 20 days. RESULTS: Electron microscopy showed that the chemotherapy-induced damages to the epididymal epithelia mainly included cell swelling, local cavitation of mitochondria, tumor-like change in nucleoli, agglutination of marginal translocation of heterochromatin and cell apoptosis. The damage to the epithelial ultrastructure was slight in the treatment group as compared with the model rats. Chemotherapy significantly affected sperm concentration, sperm viability and sialic acid (SA), which were (15.59 +/- 4.01) x 10(6)/ml, (76.71 +/- 10.11)% and (19.38 +/- 9.34) g/mg prot in the model group in comparison with (10.63 +/- 3.82) x 10(6)/ml (P < 0.01), (60.03 +/- 7.54)% (P < 0.01) and (13.62 +/- 7.81) g/g prot (P < 0.05) in the control. Epimedium significantly increased sperm viability in the treatment group (60.03 +/- 7.54)% as compared with the model rats (69.90 +/- 12.58)% (P < 0.05). CONCLUSION: Epimedium can lessen chemotherapy-induced damage to the epididymis and protect the reproductive function of rats.