| 三氧化二砷诱导血管内皮细胞凋亡和抑制血管生成的研究 |
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| 引用本文： | 喻智勇,王曙光,李昆,陈莉. 三氧化二砷诱导血管内皮细胞凋亡和抑制血管生成的研究[J]. 西南国防医药, 2003, 13(4): 363-365 |
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| 作者姓名： | 喻智勇 王曙光 李昆 陈莉 |
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| 作者单位： | 第三军医大学西南医院全军肝胆外科研究所,重庆,400038 |
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| 摘 要： | 目的：研究三氧化二砷(As2O3)抑制血管内皮细胞(HUVECs)生长、诱导凋亡和抑制血管生成的作用。方法：As2O3与HUVECs共同孵育一定时间后，观察细胞形态学变化，并用MTT方法测定细胞生长曲线图、TUNEL方法检测细胞凋亡；观测As2O3对鸡胚血管生成的影响。结果：在0．75μmol／L～6μmol／L浓度范围内随药物浓度增加或作用时间延长，细胞生长抑制和细胞凋亡效果越显著；As2O3作用鸡胚绒毛尿囊膜后血管生成减少。结论：As2O3抑制HUVECs生长、诱导细胞凋亡，对血管生成有抑制作用。
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| 关 键 词： | 三氧化二砷 诱导 血管内皮细胞 血管生成 细胞凋亡 细胞形态学 MTT 急性早幼粒细胞白血病 |
| 修稿时间： | 2003-03-25 |
Study on the apoptosis of human vascular endothelium cells and inhibition of angiogenesis induced by arsenic trioxide |
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| YU Zhi - yong,WANG Shu - guang,LI Kun,CHEN Li. Research Institute of Hepatobiliary,Southwest Hospital of Third Military Medical University,Chongqing .. Study on the apoptosis of human vascular endothelium cells and inhibition of angiogenesis induced by arsenic trioxide[J]. Medical Journal of National Defending forces in Southwest China, 2003, 13(4): 363-365 |
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| Authors: | YU Zhi - yong WANG Shu - guang LI Kun CHEN Li. Research Institute of Hepatobiliary Southwest Hospital of Third Military Medical University Chongqing . |
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| Affiliation: | YU Zhi - yong,WANG Shu - guang,LI Kun,CHEN Li. Research Institute of Hepatobiliary,Southwest Hospital of Third Military Medical University,Chongqing 400038. |
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| Abstract: | Objective: To study the effect of arsenic trioxide (As2O3) on human vascular endothelium cells (HUEVCs) and angiogenesis. Methods: As2O3 and HUEVCs were incubated together for some time to observe the cytomorphological changes. To observe the effect of As2O3 On the angiogenesis of chick - embryo vascular, MTT reduction assay was used to detect the cytogrowth curve and TUNEL was employed to measure the cell proliferation. Results: In the concentration range of 0.75 - 6 umol/L, As2O3 - induced cell apopto-sis and As2O3 - inhibited angiogenesis were enhanced in time - and - concentration - dependent manner. What' s more, As2O3 significantly inhibited the angiogenesis in chorio - allantioc membrane (CAM). Conclusion: As2O3 can inhibit the growth of HUEVCs, induce the apoptosis of cells and reduce the angiogenesis in CAM. |
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| Keywords: | As2O3 endolhelial cell apoptosis angiogenesis. |
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