| 血栓靶向脂膜超声造影剂的制备与初步评价 |
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| 引用本文： | 夏红梅,高云华,刘政,谭开彬,杨钰楠,杨莉,钱频,王冬. 血栓靶向脂膜超声造影剂的制备与初步评价[J]. 中国医学影像技术, 2008, 24(2): 176-179 |
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| 作者姓名： | 夏红梅 高云华 刘政 谭开彬 杨钰楠 杨莉 钱频 王冬 |
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| 作者单位： | 重庆第三军医大学新桥医院超声科,重庆,400037 |
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| 摘 要： | 目的制备血栓靶向脂膜超声造影剂,并对其配体结合率进行初步评价。方法通过生物素-抗生物素蛋白桥连法,制备携带血栓靶向配体的脂膜超声造影剂,非靶向脂膜微泡为对照;采用流式细胞仪初步评价靶向微泡配体结合率及其影响因素。结果靶向微泡荧光检测为阳性,对照组为阴性;流式细胞仪分析结果显示该血栓靶向微泡配体结合率达82.96%,对照组仅为0.92%、0.89%。结论采用生物素-抗生物素蛋白桥连接法,可以成功制备血栓靶向脂膜超声造影剂。微泡纯化过程中离心速度与离心时间对配体结合率的影响具有显著意义。
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| 关 键 词： | 血栓靶向 脂质体 超声造影剂 血栓靶向 脂膜 超声造影剂 评价 assessment initial ultrasound contrast agent 意义 影响因素 离心时间 离心速度 纯化过程 连接法 显示 流式细胞仪分析 阴性 对照组 阳性 荧光检测 结果 |
| 文章编号： | 1003-3289（2008）02-0176-04 |
| 收稿时间： | 2007-12-11 |
| 修稿时间： | 2008-01-09 |
Preparation of thrombus-targeted lipid-coated ultrasound contrast agent and initial assessment |
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| XIA Hong-mei,GAO Yun-hu,LIU Zheng,TAN Kai-bin,YANG Yu-nan,YANG Li,QIAN Pin and WANG Dong. Preparation of thrombus-targeted lipid-coated ultrasound contrast agent and initial assessment[J]. Chinese Journal of Medical Imaging Technology, 2008, 24(2): 176-179 |
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| Authors: | XIA Hong-mei GAO Yun-hu LIU Zheng TAN Kai-bin YANG Yu-nan YANG Li QIAN Pin WANG Dong |
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| Affiliation: | Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China;Department of Ultrasound, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China |
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| Abstract: | |
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| Keywords: | Thrombus target Liposome Contrast media |
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