BCPT对慢性应激抑郁大鼠下丘脑、垂体AVP含量及下丘脑AVP mRNA表达的影响

目的观察一种拟青霉代谢产物提取物(BCPT)对慢性应激抑郁大鼠行为,下丘脑、垂体精氨酸加压素(AVP)含量及下丘脑AVP mRNA表达的影响。方法采用慢性不可预见性应激造成大鼠抑郁模型,运用开野(Open-F ield)法测定动物行为,用放射免疫分析方法(R IA)测定慢性应激抑郁模型大鼠下丘脑、垂体AVP含量,用半定量逆转录-聚合酶链反应(RT-PCR)方法,测定其下丘脑AVP mRNA的表达。结果BCPT可提高慢性应激大鼠的水平和垂直活动数,降低动物下丘脑、垂体AVP含量,下调下丘脑AVP mRNA表达。结论BCPT的抗抑郁作用可能与降低慢性应激大鼠下丘脑、垂体AVP含量,下调下丘脑AVP mRNA表达有关。

Effect of BCPT on AVP content of hypothalamus and pituitary and AVP mRNA of hypothalamus in chronic stress rats

Aim To study the effect of bioactive compounds from paecilomyces tenuipes(BCPT) on AVP content of hypothalamus,pituitary and expression of AVP mRNA of hypothalamus and behaviour in chronic unpredictable stress model of depression in rats.Methods The depression animal model was induced by chronic unpredictable stress.The behaviour of rats was tested in the open field.The effect of BCPT on AVP content in hypothalamus and pituitary was tested by radioimmunoassay.RT-PCR was used to test the expression of AVP mRNA in hypothalamus.Results BCPT could decrease the expression of AVP mRNA of hypothalamus and decrease AVP content of hypothalamus and pituitary in chronic stressed rats obviously.BCPT could increase ambulation and rearing score of chronic stressed rats in the open-field test.Conclusion BCPT exhibited an antidepressant-like effect may in part be associated with the decreasing AVP content of hypothalamus and pituitary,and the expression of AVPmRNA of hypothalamus in chronic unpredictable stress model of depression in rats