| IL34转基因小鼠的建立和表型分析 |
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| 引用本文： | 杨青,全雄志,廉虹,张旭,马春梅,张连峰. IL34转基因小鼠的建立和表型分析[J]. 中国比较医学杂志, 2011, 21(6): 64-66,59,90. DOI: 10.3969/j.issn.1671.7856.2011.06.014 |
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| 作者姓名： | 杨青 全雄志 廉虹 张旭 马春梅 张连峰 |
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| 作者单位： | 中国医学科学院,北京协和医学院,医学实验动物研究所,卫生部人类疾病比较医学重点实验室,国家中医药管理局人类疾病动物模型三级实验室,北京100021 |
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| 基金项目： | 卫生部项目;实验动物和人类疾病动物模型资源扩展，十一五新药专项支持 |
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| 摘 要： | 目的建立白介素34转基因小鼠,研究该基因在小鼠中表达对小鼠免疫系统的作用。方法把人的IL34基因插入CMV启动子下,构建转基因表达载体,通过显微注射法建立IL34转基因C57BL/6J小鼠。PCR鉴定IL34转基因小鼠的基因表型,Western blotting检测IL34蛋白的表达水平,组织化学染色观察IL34转基因小鼠重要器官的病理改变。结果建立了2个品系的IL34转基因小鼠。转入的人IL34基因在脾脏中的表达水平高于内源性IL34。组织学分析显示IL34转基因小鼠各重要器官如脑,心,肝,肾,肠等的形态结构均正常,但脾脏的生发中心比较活跃,白髓的范围比野生型小鼠大。结论成功建立了IL34转基因小鼠,IL34基因的过度表达对免疫系统作用需要进一步探讨。
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| 关 键 词： | 白介素34 转基因小鼠 巨噬细胞 |
The Establishment of Interleukin 34 Transgenic Mice |
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| YANG Qing,QUAN Xiong-zhi,LIAN Hong,ZHANG Xu,MA Chun-mei,ZHANG Lian-feng. The Establishment of Interleukin 34 Transgenic Mice[J]. Chinese Journal of Comparative Medicine, 2011, 21(6): 64-66,59,90. DOI: 10.3969/j.issn.1671.7856.2011.06.014 |
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| Authors: | YANG Qing QUAN Xiong-zhi LIAN Hong ZHANG Xu MA Chun-mei ZHANG Lian-feng |
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| Affiliation: | YANG Qing,QUAN Xiong-zhi,LIAN Hong,ZHANG Xu,MA Chun-mei,ZHANG Lian-feng(Key Laboratory of Human Disease Comparative Medicine,Ministry of Heath,Institute of Laboratory Animal Science,Chinese Academy of Medical Sciences & Peking Union Medical College,Human Disease Animal Models Level-3 Laboratory of State Administration of Traditional Chinese Medicine,Beijing 100021,China) |
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| Abstract: | Objective To generate the interleukin 34(IL34) transgenic mice and investigate its functions on mouse's immune system.Method The transgenic plasmid was constructed by inserting the human IL34 gene in the downstream of CMV promoter.The transgenic mice were produced by microinjection method and the genotype was detected by PCR.The expression level of the protein was determined with Western blotting.The pathologic changes of important organs were observed by histochemical stain.Result Two lines of transgenic C... |
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| Keywords: | Interleukin 34 Transgenic mice Macrophage |
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