| 细胞色素P450基因对高脂饮食诱导不同周龄小鼠动脉粥样硬化的保护作用机制 |
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| 引用本文： | 吴俊,陈琛,曾和松,汪道文.细胞色素P450基因对高脂饮食诱导不同周龄小鼠动脉粥样硬化的保护作用机制[J].中国分子心脏病学杂志,2011,11(2). |
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| 作者姓名： | 吴俊 陈琛 曾和松 汪道文 |
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| 作者单位： | 1. 长江航运总医院检验科,武汉,430010
2. 华中科技大学同济医学院附属同济医院心血管内科,武汉,430030 |
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| 摘 要： | 目的在高脂饮食诱导小鼠动脉粥样硬化中研究CYP2C8基因对动脉粥样硬化的作用及相关机制。方法 :以20和40周龄APOEKO+/-CYP2C8Tg+/-和CYP2C8Tg+/-转基因的小鼠为研究对象,相同基因背景和周龄的同窝APOEKO+/-和C57BL/6小鼠设为对照。在第5,20,40周时测各组动物EET含量,用Real-time PCR法检测各组小鼠的主动脉PPAR-γ和MCP-1基因表达水平,Western blot分析各组小鼠的主动脉(p-)eNOS和NF-κB蛋白的表达与小鼠的肝脏PI3K、(p-)AKT、(p-)ERK和(p-)P38等信号通路蛋白的表达情况。结果 :在高脂饮食中,CYP2C8Tg+/-组和APOEKO+/-CYP2C8Tg+/-组的EET浓度明显增高。Western blot显示:高表达CYP2C8在肝脏中明显上调PI3K、(p-)AKT、(p-)ERK和(p-)P38蛋白;在主动脉明显上调(p-)eNOS蛋白,下调NF-κB蛋白。Real-time PCR分析显示高表达CYP2C8在主动脉中下调MCP-1蛋白。结论 :本研究高表达CYP2C8基因具有显著的抗炎作用,这一作用是...
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| 关 键 词： | 细胞色素P450 环氧化二十碳三烯酸 动脉粥样硬化 炎症 基因治疗 |
Mechanisms of Arachidonic Acid Cytochrome P450 Epoxygenase on Atherosclerosis Induced by Western Diet in Different Week-old Mice Model |
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| WU Jun,CHEN Chen,ZENG He-song,WANG Dao-Wen.Mechanisms of Arachidonic Acid Cytochrome P450 Epoxygenase on Atherosclerosis Induced by Western Diet in Different Week-old Mice Model[J].Molecular Cardiology of China,2011,11(2). |
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| Authors: | WU Jun CHEN Chen ZENG He-song WANG Dao-Wen |
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| Institution: | WU Jun; CHEN Chen; ZENG He-song; WANG Dao-Wen.Department of Internal Medicine; Tongji Hospital; Tongji Medical College; Huazhong University of Science and Technology; Wuhan 430030; China; General Hospital of the Yangtze River Shipping; Wuhan; 430010; |
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| Abstract: | Objective This study investigates therapeutic effects of human tissue CYP2C8 gene expression on atherosclerosis and the possible mechanisms. Methods: Overexpression of CYP2C8 genes in endothelium of 20-week-old and 40-week-old of APOEKO+/- CYP2C8Tg+/-and CYP2C8Tg+/- mice were identified using PCR. The same aged APOEKO+/- and C57BL/6 mice were served as control (n=10). At 5, 20 and 40 weeks, urine EET were measured by ELISA. Aorta PPAR-γ and MCP-1 were measured by quantitative real-time PCR. The expressions ... |
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| Keywords: | Arachidonic Acid Cytochrome P450 Epoxygenase Epoxyeicosatrienoic Acids Atherosclerosis inflammation Gene Therapy |
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