| 血管内皮细胞对平滑肌细胞表型转化的影响 |
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| 引用本文： | 李晓聪,刘水,潘銮凤,吴国强. 血管内皮细胞对平滑肌细胞表型转化的影响[J]. 中国分子心脏病学杂志, 2011, 11(2) |
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| 作者姓名： | 李晓聪 刘水 潘銮凤 吴国强 |
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| 作者单位： | 1. 复旦大学上海医学院分子生物学实验室,上海,200032
2. 复旦大学力学与工程学系,上海,200433 |
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| 摘 要： | 目的分析共培养时大鼠血管内皮细胞(VECs)对大鼠血管平滑肌细胞(VSMCs)表型转化的影响。方法 VSMCs直接种植于培养板表面,VECs则种植于与VSMCs相对的浮胶底面。免疫荧光方法观察和鉴定VECs和VSMCs,RT-PCR和共聚焦显微镜分析表型相关基因的表达。结果原代培养的大鼠VECs呈铺路石样形态,vWF染色阳性。原代培养的大鼠VSMCs免疫荧光染色α-SMA呈阳性。RT-PCR检测结果表明,48h共培养组VSMCs的合成表型相关基因CRBP-1、Smemb的表达水平显著高于单独培养组,分别为1.4倍、1.5倍;72h达到峰值,分别为1.7倍、2.1倍,96h开始下降;共培养组中收缩表型标记物Smoothelin-B和SM-MHC的基因表达水平在48h、72h显著低于单独培养组,96hSmoothelin-B却高于单独培养组。单独培养组上述各基因的变化趋势不变或保持稳定。免疫荧光结果显示SM-MHC蛋白表达在共培养组中96h后从下降转为升高(P<0.05)。结论在共培养体系中,血管内皮细胞对血管平滑肌细胞表型转化的作用表现为先促进向合成型转化,96h后促进向收缩型转化。
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| 关 键 词： | 表型 细胞共培养 血管内皮细胞 血管平滑肌细胞 基因表达 |
Effect of Vascular Endothelial Cells on Phenotypic Transition of Smooth Muscle Cells |
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| Li Xiao-cong,Liu Shui,Pan Luan-feng,Wu Guo-qaing. Effect of Vascular Endothelial Cells on Phenotypic Transition of Smooth Muscle Cells[J]. Molecular Cardiology of China, 2011, 11(2) |
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| Authors: | Li Xiao-cong Liu Shui Pan Luan-feng Wu Guo-qaing |
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| Affiliation: | Li Xiao-cong1; Liu Shui1; Pan Luan-feng1; Wu Guo-qaing2. 1 Laboratory of Molecula Biology; Shanghai Medical College; Fudan University; Shanghai; 200032; China; 2 Department of Mechanics and Engineering; 200433; China.; |
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| Abstract: | Objective To observe the influence of vascular endothelial cells(VECs) on phenotype alternation of vascular smooth muscle cells (VSMCs). Methods VECs and VSMCs were isolated from thoracic aortas of rat using collagenase digestion and tissue section culturing respectively. A single layer of VSMCs were cultured on the downside of floating gel of Type I collagen from rat tails and faced a single layer of VECs attaching on the surface of cell culture plate. The crosstalk between VECs and VSMCs was allowed throu... |
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| Keywords: | Phenotype Co-culture vascular smooth muscle cells vascular endothelial cells gene expression |
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