NGF在人胚胎脊髓中的表达及变化

目的探讨神经生长因子(NGF)在人胚胎脊髓胸段中的表达及变化.方法从各时间段(6周、8周、10周、3月、4月、5月、6月流产胚胎中取出脊髓后,用免疫组织化学和RT-PCR方法检测NGF的抗原和mRNA表达情况及变化规律.结果免疫组织化学检测到在胸段脊髓的灰质处有NGF-IR的分布;RT-PCR均检测到NGF的mRNA在胸段脊髓表达,从6周开始上调,在8周有一个高峰,10周~6月整体趋于稳定.结论在人早、中期胚胎脊髓中NGF均有表达,说明胚胎脊髓发育和NGF存在一定的关系.

The Expressions and Changes of NGF in the Fetal Human Spinal Cord

Objective To investigate the expressions and changes of NGF and in pars thoracica medullae spinalis of the fetal human spinal cord.Methods Spinal cord was taken out from elective abortion foetus at different period of 6 week,8 week,10 week,3 month,4 month,5 month and 6 month respectively,then immunohistochemistry and RT-PCR were used to detect the change law of NGF expression.Results NGF-IR could be seen respectively in gray matter in pars thoracica medullae spinalis of the fetal human spinal cord from 6 week to 6 month by immunohistochemistry method.And the expression of NGF's mRNA was detected by RT-PCR and there was increases from week 6 and peak in 8 week and there was total stability from 10 week to 6 month.Conclusion There is NGF expression in the early and middle stages of fetal human spinal cord,which shows that there must be some relations between the embryonic development and NGF.