Sandwich enzyme immunoassay for human interleukin-1 alpha produced in vitro by peripheral blood mononuclear cells |
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| Authors: | K Tanaka E Ishikawa Y Ohmoto Y Hirai |
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| Affiliation: | Department of Biochemistry, Medical College of Miyazaki, Japan. |
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| Abstract: | We describe a new high pressure liquid chromatography (HPLC) method for quantitative determination of urinary lysozyme. The method is simple, reproducible and with detection limit of 0.2 microgram. Before HPLC analysis the urine was purified using a Sep-Pak C18 cartridge. Lysozyme concentration was significantly higher in patients with chronic renal failure than in a control group (p less than 0.001). The concentration of lysozyme in urine is shown to be a sensitive indicator of renal damage. |
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